The different bivalent cations with the same final concentration were put into the dnaase reacting system for 15 minute at ph5 . 4 , at 41 , then dnaase ' s activity unit was detected 在dna酶的酶促反应体系中加入终浓度相同的二价金属阳离子41下、 phs 4准确反应15分钟后测定酶的活力单位。
A charged mosaic membrane with ideal selectivity has apparently advantage over the conventional nanofiltration ( nf ) membrane , which can be used to concentrate the monovalent salts and bivalent salts 我们知道，传统的纳滤膜仅仅能透过一价盐，而在化学工业中对于二价盐的选择性传递有时尤为重要。
On the basis of distinctions of verbal valency , verbs used within oracle inscriptions can be divided into the following four categories : zero - valent verbs , monovalent verbs , bivalent verbs and trivalent verbs 摘要甲骨文中的动词可以根据其配价的不同分成以下四类，即零价动词、一价动词、二价动词、三价动词。
In general , bu is used to negate non - verbs , zero - valent verbs and monovalent verbs , while though fu is often used to negate bivalent verbs and trivalent verbs , bu can also be used to negate these two verb types 一般说来，非动词、零价动词、一价动词要用不来否定，而二价动词、三价动词常用弗来否定，但也可以用不来否定。
On the basis of catalyzing mechanism , it can raise the reaction efficiency of sodium sulfite and reduce the remains of sodium sulfite , and has evident catalyzing action by adding bivalent nickel ions and bivalent cobalt ions into the water under the condition of weak alkali 根据催化机理，在弱碱条件下加入二价镍离子和二价钴离子能提高水中亚硫酸钠的反应效率，降低水中亚硫酸钠的残余量，对亚硫酸钠的除氧效果具有明显催化作用。
The growing graphs of e . coli bl21 containing pbv221 and pbv221svhb according to the od600 values showed that the e . coli vgb + grew faster 4 the highly - efficient bivalent vectors which are named pgbif4abcvhb and pgbi4asvhbbt respectively , havfs been . su ? essful ] y constructed . the tobacco leave discs were transformed with agrobacterium tumefaciens carrying the plant expression vectors pgbif4abcvhb and pgbi4asvhbbt . 38 and 36 transgenic tobacco plants have been obtained respectively after kanamycin screening , pcr detection , southern blot analysiss showed that the foreign genes have been inserted into the tocacco plant genome . the expression of 16kd vhb protein in transgenic plants was proved by western blot 4构建了双价基因高效植物表达载体pgbif4abcvhb和pgbi4asvhbbt 。通过农杆菌介导法，将这两个双价基因高效表达载体导入烟草，分别获得了38 、 36株阳性转基因植株。经pcr 、 southernblot分子生物学方法分析，证明vgbm ， bt基因、 bt - cpti基因已整合到烟草基因组中， westernblot证明vgbm成功地表达出16kd的vhb蛋白。
The experimental results show that the cycle of the mother liquid increases the yield of the product , the seed crystal can improve long - diameter ratio remarkably , the proper additive reduces the speed of crystal growth and make size distribution homogenize . through appending different seed crystal and bivalent lead at one time , we may receive when the content of the seed crystal is 0 . 075 % and pb2 + is 20 - 50ppm , the yield of the basic magnesium chloride whisker is high and crystal shape is good . the surface active agent can improve dispersion performance of the basic magnesium chloride whisker 研究结果表明：母液循环可以提高碱式氯化镁晶须的产率；添加晶种可明显的改善晶形；合适的添加剂可以降低晶体生长速度并可提高晶须的粒径分布；而同时加入不同用量的晶种和pb ~ ( 2 + ) ，试验结果表明晶种含量在0 . 075 ， pb ~ ( 2 + )在20 50ppm时，晶须的产率高，晶形好；表面活性剂对产品的分散性能有所改进。
In order to investigate the effects of ph , temperature , naf and bivalent cations on the conformation of the phosphatase in solution , we monitored the difference of intrinsic fluorescence of the phosphatase and compared changes of the enzyme activity under those conditions . the tertiary structure loosed and the intensity of fluorescence decreased below ph 6 . 0 . the intensity of fluorescence was lowest at ph 5 . 0 and the tertiary structure was reconstructed as the solution ph was increased 其中在ph5 . 0及以下时，蛋白质的三级结构变得松散，荧光强度下降， ph5 . 0时尤为显著，而当溶液ph高于5 . 0时(酶的最适ph ) ，样品的荧光发射强度明显增加，表明酶蛋白受溶液酸碱度的影响，构象发生部分变化，部分trp残基向疏水环境移动，其三级结构得到恢复。
In this article , the highly - efficient plant vectors with bivalent genes have been constructed by combining the gene of vhb with bt gene and cfmcryla - cpti gene respectively , studying the influence over the plants metabolic level and growth . we manage to reach the goal of increasing the yield of transgenic plants and cultivate new plant varities with pest - resistance by applying transgenic approaches , and eventually in the hope of cultivating new varities of crops with hoth highly - steady - yield and pest - resistance 本文尝试将透明颤菌血红蛋白( vhb )基因优化、人工合成并且分别和gfmcryia ( bt )基因、融合杀虫基因( bt - cpti )构建成双价基因植物高效表达载体，导入烟草，研究该基因对植物代谢水平和植物生长的影响，尝试通过基因工程育种技术增加转基因作物产量的同时，双可使其具有抗虫性，以期望培育出高产、稳产又具有抗虫效果的新品种。
The fluorescence intensity of the phosph atase increased , followed by a decreasing process , in the temperature region of 40 - 90 c . the enzyme was inhibited by naf significantly and the fluorescence spectra of the protein were influenced obviously by the concentration of naf . the data from our experiments showed that the bivalent cations can modulate the activity of the phosphatase but maybe is independent of the tertiary structure Edta对此种磷酸酣酶也有抑制作用，因此用7种二价阳离子处理磷酸酷酶，发现不同金属二价阳离子对这种磷酸酷酶活性和荧光发射光谱的影响不同，因此二价阳离子调控此酶活性，但可能与荧光光谱所反映的三级结构变化无关。
