Aliquots of cells were mixed 0 . 15 % mg / ml fb - 28 , and kept at 4c for 30min , fusion assays were conducted : fluorescence was measured immediately at regular time - points with fluorescence spectrophotometer with an excitation wave length of 560nm and emission wave length of 590nm . the percentages of membrane fusion was calculated . by monitoring fusion using the r18 assay , we found that the fluorescent brightener 28 influenced membrane fusion of virus and midgut epithelia cells 此外，采用分子探针r18 (荧光标记物)标记病毒囊膜，体外分离中肠上皮细胞，将标记的病毒粒子与离体中肠上皮细胞混合后保温，病毒吸附zh后，通过检测荧光的变化来监测病毒粒子与上皮细胞的融合。
The foci located the basal in midgut not in control but also in test , the results showed that the addition of optical brighteners did n ' t change the spots of infection but improved the viral enhancement , in addition , infection of hemolymph cell showed that optical brightener increased sensitivity of larvae to virus , forming more foci 可见，对于处于不同时龄的供试幼虫接种病毒时，添加荧光增白剂均可提高病毒在中肠组织中的感染能力。从病毒在中肠中形成的病灶部位可以看出，实验组与对照组的病灶均靠近中肠的基底膜，荧光增白剂的加入并不能改变病毒在中肠组织的感染部位。
The mechanism enhancement of the optical brightener is not known . shapiro et al . postulated that selected brightener including m2r inhibit or alter the chitinous peritrophic membrane ( pm ) , creating gaps in the membrane or gut lining and perhaps allowing more virions to pass from the gut lumen into the hemocoel 光增白剂对杆状病毒的增效作用的机理存在两种推测一种观点认为光增白剂是通过破坏围食膜结构的完整性，促使更多的病毒粒子穿越围食膜而发动感染的；另一种意见认为光增白剂能延迟中肠上皮细胞的脱落，促进病毒的复制繁殖。
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