The results showed that the total coincidence rate between digfa and sat and the coincidence on positive rate of detection were 99 . 88 % ( 4003 / 4008 ) and 94 . 25 % ( 82 / 87 ) , respectively , and the sensitivity of digfa appeared to be higher in population with brucellosis and in high - titered serum samples 方法用已建立的布病滴金免疫测定技术检测不同职业和不同感染类型人员的布病抗体，并与布病试管凝集试验作平行检测。
These results demonstrate that the dot - immunogold assay shows its practical value to monitor the brucella infections among the epidemic area and individuals with different occupations in the un - epdemic area , and this method can also be applied for the clinical investigation and epidemiological survey of brucellosis 结论滴金免疫测定法对布病疫区人群和非疫区重点职业人员开展布病监测具有实用价值，也可用于布病的临床检测和流行病学筛查。
Experiment shows that the antigen extracted by autoclave is best for dot - ppa - elisa . applying the x2 test , optimal concentration of antigen was determined , and the optimum dilution of enzyme hrp - spa was 1 : 10 . the high specificity of dot - ppa - elisa was proved by the specific blocking test , and also by the cross - reaction test in which the diaphragm does not react with the antibodies against salmonellosis , pasteurellosis , chlamydiosis , hcv , ppv , brucellosis , erysipelas suis , colibacillosis , prv 试验中对多种方法提取的诊断抗原进行了比较、筛选；确定了dot - ppa - elisa的最佳工作条件；测定了st - 171株的免疫猪血清抗体及c群、 2型人工感染猪血清抗体效价，并初步确定了该方法的阳性标准。
The high specificity of dot - ppa - elisa was proved by the specific blocking test , and also by the cross - reaction test in which the diaphragm did n ' t react with the antibodies against pasteurellosis , streptococcosis , colibacillosis , chlamydiosis , hcv , ppv , brucellosis , prv and foot - mouth disease . the diaphragm has good sensitivity and could detect some salmonella - positive test serum which has been diluted to 1 : 2048 . stored at 4 for at least 6 months or at 10 - 25 " c for 4 months , the sensitivity and specif icity of the diaphragm did n ' t change , so it has good stability 本研究制备的诊断膜片特异性强：不与猪衣原体病、猪口蹄疫病、猪大肠杆菌病、猪布氏杆菌病、猪瘟、猪伪狂犬病、猪细小病毒病、猪巴氏杆菌病、猪链球菌病的阳性血清发生交叉反应；诊断膜片具有良好的敏感性，能够检测到1 ： 2048稀释的动物试验阳性血清；膜片的保存期长，在10 25可保存4个月、 4条件下至少可保存6个月其灵敏度不变。
Class infectious diseases shall include viral hepatitis , bacillary and amebic dysentery , typhoid and paratyphoid , aids , gonorrhea , syphilis , poliomyelitis , measles , pertussis , diphtheria , epidemic cerebrospinal meningitis , scarlet fever , epidemic hemorrhagic fever , rabies , leptospirosis , brucellosis , anthrax , epidemic and endemic typhus , epidemic encephalitis b , kala - azar , malaria , and dengue fever 乙类传染病是指：病毒性肝炎、细菌性和阿米巴性痢疾、伤寒和副伤寒、艾滋病、淋病、梅毒、脊髓灰质炎、麻疹、百日咳、白喉、流行性脑脊髓膜炎、猩红热、流行性出血热、狂犬病、钩端螺旋体病、布鲁氏菌病、炭疽、流行性和地方性斑疹伤寒、流行性乙型脑炎、黑热病、疟疾、登革热。
B class infectious diseases shall include viral hepatitis , bacillary and amebic dysentery , typhoid and paratyphoid , aids , gonorrhea , syphilis , poliomyelitis , measles , pertussis , diphtheria , epidemic cerebrospinal meningitis , scarlet fever , epidemic hemorrhagic fever , rabies , leptospirosis , brucellosis , anthrax , epidemic and endemic typhus , epidemic encephalitis b , kala - azar , malaria , and dengue fever 乙类传染病是指：病毒性肝炎、细菌性和阿米巴性痢疾、伤寒和副伤寒、艾滋病、淋病、梅毒、脊髓灰质炎、麻疹、百日咳、白喉、流行性脑脊髓膜炎、猩红热、流行性出血热、狂犬病、钩端螺旋体病、布鲁氏菌病、炭疽、流行性和地方性斑疹伤寒、流行性乙型脑炎、黑热病、疟疾、登革热。
Animal health analysis methods - control protocol of reagents control protocol of reagents for the detection of antibodies directed against brucellosis by the rose bengal test , the complement fixation test or the seroagglutination test in serum samples or for the detection of antibodies directed against bovine brucellosis by the ring test in bulk tank milk samples 动物健康分析方法.用四碘四氯荧光素试验补体结合试验或血清凝集试验检测血清样品中抗布鲁氏菌病的抗体或用环试验检测收集槽牛奶样品中抗牛布鲁氏菌的抗体用试剂的试剂控制协议的控制协议
The high specificity of the dot - ppa - elisa was confirmed by specific blocking test and also by cross - reaction test . the diaphragm did not react with the antibodies against salmonellosis , streptococcosis , colibacillosis , chlamydiosis , hcv , ppv , prv , brucellosis . erysipelas , suis and chlamydiosis in cross - reaction test . the diaphragm has good sensitivity and could detect some pasteurella - positive test serum which has been diluted to2 - " 试验证明所建立的dot - ppa - elisa具有较好的特异性，与猪瘟、仔猪副伤寒、猪丹毒、猪细小病毒病、猪伪狂犬、猪布氏杆菌病、猪衣原体病阳性血清无交叉反应。
The epidemiological investigation of deer brucellosis in part of province and area demonstrated that brucellosis was universal in raising sika deer and wapiti in china , the positive ratio in female deer was higher than male deer ; the positive ratio respectively was 10 . 06 % 、 4 . 77 % in sika deer and 11 . 15 % 、 4 . 99 % in wapiti 摘要对我国部分省区鹿布氏杆菌病进行流行病学调查表明，我国鹿场饲养的梅花鹿、马鹿群中普遍存在鹿布氏杆菌病，母鹿阳性率明显高于公鹿，梅花鹿阳性率分别为10 . 06 % 、 4 . 77 % ；马鹿阳性率分别为4 . 90 % 、 11 . 15 % 。
