These studies demonstrates that not only we could make transgenic mouse embryo with high efficiency by the method of icsi , but also the fresh sperm from cauda epididymis or freeze - thawed dead sperm pre - mixed with exogenous dna had the full development potential to develop into term 本实验充分表明， icsi精子载体法可以比较高地制造小鼠转基因胚胎，小鼠附睾新鲜精子及无抗冻剂保护冷冻致死精子都具有足够的全程发育潜力。
Car tracing cauda is a bad traffic safety accident in speedway . more and more people pay attention to it . in recent years people try to solve car elude tracing cauda in theories and technologies . and building tracing cauda model is the fundation for studing car tracing cauda . after analyzing causation of car tracing cauda , and with the neural network theory , this paper gives backpropagation neural network model for solving it , and disccuses a technic scheme for car tracing cauda prediction system , simulation shows the bp neural network model is acceptable 高速公路汽车追尾是一种严重的交通安全事故，引起了交通科技界的广泛重视.近几年来，人们试图从理论与技术上解决高速公路汽车追尾问题，其中建立追尾的数学模型是研究追尾现象的基础.文中在分析了汽车追尾的原因后，基于神经网络理论，建立了追尾的bp网络模型，并讨论了追尾预测系统的技术实现方案
Methods of 14 normal male japanese white rabbits ( aged approximately 4 months ) , 7 ( randomly chosen ) were subjected to vasal ligation near the cauda epididymidis after exposure of the testis and vas deferens via a scrotal incision , and the other 7 subjected to vasectomy after exposure of the vas in the inguinal canal through a small ventral midline incision 方法正常雄性日本大耳白兔14只（约4月龄） ， 7只（随机选择）经阴囊切口暴露睾丸和输精管后靠近附睾尾进行输精管结扎，另7只从耻骨联合上方的腹正中线上作一小切口，暴露腹股沟管内的输精管后进行输精管结扎。
