Construction of an expressing vector for antisense rna - ribozyme chimeric dna sequence against tomato acc synthase gene and transformation of tomato 核酶嵌合基因植物表达载体的构建及对番茄的转化
Using confocal laser scanning microscopy ( clsm ) , we observed the distribution of chimeric protein in different kinds of tissues and cells in transgenic toreniafournieri 经激光共聚焦显微镜观察了转基因植株的各种不同组织中融合蛋白的表达和分布情况。
Megf and melittin was linked together by a linker into an chimeric dna , megfmel , and the chimeric dna was expressed by using pet30a as expression vector and e . coli as expression strain 值在slug砌l与54ug加l之间。在诱导表达各目的产物时诱导温度为30c 。
But testing human embryonic stem cells in this manner would create a chimeric animal with human dna throughout its body , a prospect many find ethically troubling 然而，如果以这种方式来测试人类胚胎干细胞特性，将会制造出全身带有人类dna的嵌合动物，许多人认为这有违伦理。
Experiment ii study on the production of porcine chimera by injecting embryonic stem cells ( eg ) . the method of chimeric production is established in this experiment 建立了本实验室条件下猪胚胎干细胞( eg细胞)嵌合体的制作方法，所用固定针的外径为130 150 m ，内径为40 m ，注射针内径为25 m 。
To investigate the molecular basis of the stresses - induced gene regulation , the acbadh promoter - / ? - glucuronidase chimeric gene constructs containing six deletions were introduce into tobacco by agrobacterium - mediated transformation 对启动子进行6禾缺夫构建，瞬时转化烟草叶片表明， 6种构建都有活性。
Britain , so far one of the least hysterical countries , recently postponed a decision about whether making chimeric cells out of rabbit - egg cytoplasms and human nuclei should be illegal 英国? ?这个迄今最不歇斯底里的国家之一，最近对是否从兔子卵细胞的细胞质中制造出嵌合体细胞以及人类细胞核是否非法推迟作出决定。
Since the cells of the immune system are derived from stem cells in the bone marrow , these patients go on to develop what is known as chimeric immunity , which blends elements from the immune systems of both the donor and the recipient 因为免疫细胞源于骨髓干细胞，这些病人产生了一种同时具有捐献者和接受者免疫系统成分的二元免疫。
In transgenic tobacco plants , the analysis of transient expression by monitoring 3 - glucuronidase activity revealed that a chimeric gene construct containing a 1 . 2 kb pdf1 . 2 promoter fused to a gus reporter gene was induced by meja ( 1 )从拟南芥基因组中扩增出长度约为1200bp的pdf1 . 2启动子，与gus构建的融合基因在烟草中的表达受meja诱导。
Observe gvhd for 60 days . the results showed that mouse lymphoid cells were present in the sd rats detected by fluorescence activated cell sorter ( facs ) for morn than 90 days . the chimeric rates were 18 % - 45 % , 7 % - 23 % , 2 % - 8 % on day 30 , 60 , 90 respectively 随着时间的推移，嵌合率逐渐下降。骨髓移植后60天及90天的嵌合率分别为7 - 23 ， 2 8 。
