In addition , the results of dna direct sequencing reveal that 1 out of 18 rifampin - resistant isolates has deletion in codons 513 and 514 of rpob , and the other 17 are all singe - point mutation in their rpob genes 此外， dna直接测序结果显示， 18株耐利福平菌株中，一株rpob基因的513 、 514位密码子发生了缺失，其它17株的rpob基因皆发生了单碱基突变。
The polyhedrin gene sequence in recombinant virus bmpak - hbmp can be more benefit to enhancing the expression of the hbsag ( pres2 + s ) from atg initial codon at the 5 " ends of pres2 gene 另一方面， elisa检测表明， presz抗原性提高率约是hbsag的14倍，提示bmpak hb帅中多角体基因部分碱基序列的存在更有利于从presz前的atg开始表达中蛋白全基因（ presz s ） 。
The high at content of the native gene is very difficult to express , we designed a new he gene by reverse the codon used for e . coli and yeast , then , the he gene was cloned into the expression vector pqe - 60 本研究尝试了其保护性抗原基因在大肠杆菌及酵母中的表达。天然bont a的hc片段基因中at含量高达76 ，其通过同义密码子置换人工合成hc段基因，将at含量降到57 。
5 ' race analysis indicated that ha 122 transcripts start predominantly in the consensus major late transcription initiation motif rtaag around 47nt upstream of the putative translation start codon with a minor start at position - 89 5 ’ race ( rapidamplificationofcdnaend )分析结果表明ha122基因的转录主要从翻译起始密码子上游第47个碱基起始，该处有一个晚期转录信号taag 。
