Previous evidence showed that an increase in [ ca2 + ] cyt has been shown to be an early event in the aba signal transduction pathway , and ca2 + channels at the plasma membrane of stomatal guard cells are activated by hyperpolarization and aba Assmann已提出较为完善的气孔保卫细胞信号转导模式，但是对于aba信号转导的一些中间环节仍是人们不断探讨的问题。
These results showed that cyt b gene is very suitable for identifying subspecies of tiger . we proposed specific haplotypes on cyt b gene for specifically identifying amur tiger , bengal tiger , indo - china tiger and sumatran tiger 这表明mtdnacytb基因非常适合虎亚种识别；本文提出了cytb基因上识别东北虎、孟加拉虎、印支虎、苏门答腊虎4个亚种的特异性单倍型。
We designed one primer pairs ctb - 1 , ctb - 2 to amplify about 580bp of cyt b gene sequence as a molecular marker to analyze phylogenetic relationship of 14 species of oedipodidae in china . dna sequences were aligned using clustal x , followed by refinement by eye based on the corresponding deduced amino acid sequences . after cutting off 5 " and 3 " termini unaligned sequences , we get 462 bp segment 本研究从分子生物学角度入手，采用cytb作为分子标记，采用自行设计的一对cytb基因特异引物ctb - 1 、 ctb - 2 ，通过pcr技术，共获得斑翅蝗科4个亚科14个种的代表个体以及癞蝗科1个种的代表个体的580bp左右的cytb部分序列。
By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ) , zymogram of 23 species of 11 genera in 4 subfamilies mirinae , orthotylinae , phylinae and deraeocorinae are gained . by specific primer pcr and sequencing , 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae , orthotylinae , phylinae , deraeocorinae , bryocorinae from 64 species of 23 genera which involved in this research , and 2 outgroup species of family anthocoridae as well . 1 首次通过特异引物扩增和基因测序的研究方法从被试的5亚科23个属的64种盲蝽中获得了盲蝽亚科、合垫盲蝽亚科、叶盲蝽亚科、赤爪盲蝽亚科和蕨盲蝽亚科bryocorinae5个亚科17个属的34种盲蝽以及外群花蝽科anthocoridae2种花蝽的cytb基因序列57条。
These results suggested that the 65kda protein was a degraded product , bioassay result showed that insecticidal activity of bl - 3 was 3 - fold higher than that of bl - 2 against 3rd instar plutelh xylostella . 3 . expression of cryla ( b ) gene in pseudom on a s fluoreseent pfx - 18 in order to study expression characteristics of icps gene in pseudom onas fluorescent , a new simple transformation method of pf was established 3 .用上述重组技术获得了含有刀口基因的lz20和含有尸2拼即“的lz20a克隆株，通过基因的诱导表达，发现p ”和pz浏民有不同的作用机制，证明了p20具有保护cyt人溶细胞的作用，这种作用并不依赖大肠杆菌受休的不同而改变
( 2 ) there are few ideal techniques for mearsuring [ ca2 + ] cyt . in conclusion , both limitation delay studies of [ ca2 + ] cyt in embryo sac during fertilization . the thesis work is to investigate the connection of [ ca2 + ] cyt and fertilization by avoiding drawbacks noted above , say , employing an ideal experimental plant , torenia fournieri , with a naked embryo sac that protrudes from the micropyle 针对这种现状，本工作选用具有裸露胚囊的玄参科的蓝猪耳植物为研究材料，研究ca ~ ( 2 + )与受精过程中相关的细胞学事件的关系，探索[ ca ~ ( 2 + ) ] _ ( cyt )在被子植物双受精过程中的作用。
In the 32 full length cyt b sequences , 14 polymorphic sites forming 10 haplotypes were found , occupying 1 . 23 % of the whole sequence . the homology among individuals is between 99 . 3 ~ 100 % , averagely 99 . 6 % , neither insertion nor deletion is found . all subspecies have their specific base at certain sites 在32个虎mtdnacytb1140bp的序列中，共发现14个位点存在变异，占总序列的1 . 23 ，个体间序列的同源性在99 . 3 100 ，平均为99 . 6 ，没有发现缺失或插入。
Sequence variation analysis exhibits high transition to transversion substitution ratios , and transitions and transversions are unsaturated in the sequence of cytochorme b gene . the base composition of the cytochrome b sequences was highly biased . using a structural model of the cytochrome b protein as a template , we divided cyt b sequence data for species within anserifomes into three functional domains 3 ）序列具有较高的转换颠换比，基因序列中的转换与颠换都未饱和，序列中的碱基组成高度偏倚： 4 ）按照细胞色素为蛋白结构模型，细胞色素b基因全序列可分为三个功能区，碱基替换饱和状况与不同功能区相关联。
A sequence of 381bp in d - loop region of 13 amur tiger mtdna were cloned and sequenced . 2 sequences of amur tiger d - loop region and 32 sequences of cyt b gene of 4 tiger subspecies in genbank were also combined to our data in the analysis of characteristics of the two regions for evaluating their significance of subspecies identification 本文克隆并测定了13只东北虎mtdnad - loop区381bp的序列，并对这些序列和genbank中注册的2个东北虎d - loop区序列和32个虎cytb基因全序列(包含4个亚种)进行了分析，研究了cytb基因和d - loop区序列的特点及其在亚种识别中的价值。
( d ) it is strongly suggested that subfamily mirinae should be revised because its remarkable heterogeneity . 3 . for the first time , cyt b gene 432bp sequences of species belonging to genera of 5 subfamilies and 2 outgroup species are obtained in this research which is more than 50 % of 64 species involved in the experiments 3 、首次在盲峙科昆虫中通过特异引物扩增和基因测序的研究方法获得了盲峙科5个亚科门个属34种盲峙和2个外群种类的cytb基因433hp序列，占被试23个属64种盲峙的50以上。
