Because of difficulty in differentiating eiav wild strain from the vaccine strain （ dla ） in vivo ， we have to build a fast identification method between wild strain and vaccine strain of eiav s2 is an accessory protein of equine infectious anemia virus ( eiav ) , the s2 function is not fully defined 由于我国马传贫弱毒疫苗存在野毒和疫苗毒鉴别困难的问题，目前尚未得到欧美等发达国家的认证，如要进入国际市场，急需建立一种eiav强、弱毒快速鉴别诊断方法。
The gene was cloned into puc19 vector and identified with pcr and eco ri + hind iii digestion . then , one of the positive recombinant clone was sequenced and analysed . the result showed that its sequence was 35 % and 32 % identical to equine ifn - and human ifn - respectively , but it only shared 15 % homology with chicken type i ifn 序列分析表明，该基因阅读开放框架由492个核苷酸组成，与马和人ifn -基因序列的同源性分别为35和32 ，与鸡i型干扰素基因的同源性仅为15 ，与国外报道的鸡ifn -基因序列完全一致。
