Moreover , together with cholesterol esterase and catalyse , cholesterol oxidase can be used as test reagent to determine the total cholesterol content in blood plasma for the diagnosis of such as cardiovascular reflex and atherosclerosis clinically 另外，在临床上胆固醇氧化酶可以与胆固醇脂酶和过氧化氢酶一起作为血浆总胆固醇含量的检测试剂，用以诊断冠心病、动脉粥样硬化等心脑血管疾病。因此，对胆固醇氧化酶的研究越来越受到重视。
General esterases of oriental migratory locust collected from the four locations were compared . general esterases were most concentrated in the thorax and abdomen , which showed 46 . 1 % and 36 . 1 % of total esterase activity in females , and 42 . 7 and 36 . 0 % in males , when using - na as substrate 东亚飞蝗中酷酶活性主要集中在胸部和腹部，水解a ~ na的酷酶活性在雌性东亚飞蝗中分别有46 . 1 %和36 . 1 %集中在胸部和腹部，在雄性中分别为42 . 7 %和36 % 。
The availability for classification of hemiptera insects by using the esterase isoenzynes , microorganisms and digestive enzymes characters has been studied . the results are as follows . 1 . there were differences in the electrophoretic pattern of the bugs 本文采用酯酶同工酶电泳、镜检与分离培养及消化酶分析对蝽类昆虫酯酶同工酶、体内微生物及主要消化酶类进行了初步研究，结果如下： 1酯酶同工酶电泳表明：蝽类昆虫酯酶同工酶谱存在明显的差异，特征酶谱重复性和稳定性较好。
According to the purity and the activity recovery , the recombinant enzyme was well purified by both of these two separation combinations . like natural gloshedobin , the recombinant enzyme exhibited strong esterase activity using tripeptide p - nitroanilide derivatives as substrate , but hydrolyzed n a - p - tosyl - l - arginine methyl ester ( tame ) or n a - benzoyl - l - arginine ethyl ester ( baee ) very weakly 与天然蛇毒类凝血酶一致，当用三肽p山itroanilide衍生物作为底物时，分泌表达的重组大连蛇岛蝗蛇毒类凝血酶具有较强的生色底物活性，但用精氨酸甲酯如tame （ na厂tosyl l ar 。
The tested isozymes included esterase ( est ) 、 superoxide dismutase ( sod ) 、 malate . dehydrogenase ( mdh ) 、 malic enzyme ( me ) 、 glutamate dehydrogenase ( gdh ) 、 peroxidase ( per ) and alcohol dehydrogenase ( adh ) . there were obvious differences between the normal strain and its degenerative strain in est 、 mdh 、 me 、 sod . the zymotype were the same in the other isozymes . the results were not only used to test the degenerative strain at young stage , but also as the theoretical base to study the genetic mechanism of the degenerative strain 而其余四种酶在正常菌株和退化菌株中酶谱一致。本研究不仅可以作为菌株退化早期鉴别的手段之一，而且为进一步研究菌种退化遗传机理提供了蛋白质水平上的一些理论依据。
The fermentation with 10l mechanically stirred fermentor was 20 hours shorter than that in the conical flasks with the highest esterase activity 14 . 6 u / ml , which was 30 % than that in the conical flasks . the optimal reaction conditions of esterase were temperature 35 and ph 9 . 0 . the esterase was stable under 40 between 5 . 0 - 9 . 0 10l发酵罐发酵与三角瓶发酵相比，培养周期缩短20h ，最大酶活达到14 . 6u ml比摇床培养酶活提高30酯酶的最适反应条件；温度为35 ， ph值为9 ，该酯酶在ph5 9及40以下的条件下比较稳定。
Most grow best and form pycnidia earlier on ma , and pda is slightly not fitful for pycnidia growth of the kind of fungi . none of the species can produce ascocarps in 60 days . esterase isoenzymes of 12 strains of rhytismataceae were studied by polyacrylamide gel electrophoresis and clustering analysis of upgma and 12 strains were classified into 4 groups when correlation coefficient is 3 . 1 结果表明大多数种在ma平板上菌落营养生长最好，产生分生抱子器及分生抱子的时间较早、能力较强； oa也是可选择的培养基之一：而p a较不适宜于该类菌物的营养生长和分生抱子器产生。
In this research , the plant esterase of wheat and - ethanoic acid naphthalene ester were taken as materials to inspect the relation between the wheat esterase and the organic phosphorus , ten linearity equations were established which expressed the esterase vigor and the organic phosphorus density logarithm with the change of the numerical value of ultraviolet resorption , and the monotony range and the extremum of the function were confirmed 摘要本研究以小麦为植物酯酶的酶源， -乙酸萘酯为底物，考察了小麦酯酶与有机磷农药之间作用的关系，建立了10个以吸光值变化表示的酶活性与有机磷农药浓度对数之间的线性方程，并确定了函数的单调区间和极值。
Based on the principle of enzyme inhibition , using the immobilized chicken liver esterase and a flow injection analysis ( fia ) enzyme biosensor ( developed by ourselves ) , this paper investigated the inhibiting power of several insecticides to the enzyme , and selected methamidophos as the standard pesticide for the quantitive determination 摘要本文基于酶抑制原理，采用固定化鸡肝酯酶和自行研制的流动注射式酶生物传感器，研究了不同农药对酶的抑制程度，确定了甲胺磷作为农药检测的定量标准。
By means of polyaerylamide gel electrophoresis , peroxidase isozyme , esterase isozyme and a - amylase isozyme of dioscorea zingiberensis , which were gathered from different growing environment conditions , were analyzed . with the results of 3 kinds of isozyme analysis and the analysis of 15 populations morphology and climate , dioscorea zingiberensis were divided into 5 ecotypes . the main results were shown as follows : 1 本研究以来自我国秦岭山脉以南的甘肃、陕西、湖北、湖南、四川及云南各省的盾叶薯蓣为试验材料，通过对盾叶薯蓣的pox 、 est和a - amy3种酶的同工酶进行聚丙烯酰胺凝胶电泳分析，结合其形态性状和物候期等生物学特征，进行生态型的划分，所得结论如下： 1
