Peripheral blood ( pb ) was collected 2 > 4 > 8 weeks after injection . facs was used to quantity human cells in the rat pb , pcr and immunohistochemisty were used to detect human cells in the pb as well as in other tissues , such as liver , spleen , pancreas , kidney , et al 1周、 2周、 4周、 8周后取新生大鼠的尾静脉血，提取基因组dna ， pcr检测人特异性_ 2微球蛋白基因序列片段；流式细胞仪检测外周血中人源cd45 ~ +细胞的比例；免疫组化检测大鼠组织中人源细胞的多向分化。
Hz cells which expressed gfp - actin fusion gene but were or were not infected by hasnpv - cl virus were checked respectively under the facs . the test showed that there is no distinctive intense difference between them . iel gene is a very early stage gene and it expresses at a low level itself . which leads to the low expression of the gfp - actin fusion gene , and so the hz cells exhibited a low intense of fluorescence 用荧光显微镜观察了融合基因的表达情况，发现gfp - actin融合基因在美国棉铃虫细胞hz中获得了成功的表达，但表达量不高；用流式细胞仪同时对表达了gfp - actin融合基因而未感染hasnpv - cl病毒的hz细胞和表达了gfp - actin融合基因而经hasnpv - cl病毒感染的hz细胞进行了荧光检测，结果表明hasnpv - cl病毒感染前后细胞中的荧光表达量没有明显的变化。
Value , and the current density and intensity versus the substorm phases . moreover , the characteristics of substorm current wedge are given special attention . it is found that the density and intensity of facs reach their peak during the expansion phase , the onset of the expansion phase is triggered when imf is changed to southward from northward or the southward imf decreases , and the positions of onset are most likely to be at the edge of plasma sheet near the earth 值分布以及场向电流强度和密度随亚暴位相的变化，并着重分析了亚暴电流楔的特征。结果表明，亚暴膨胀相的场向电流强度和密度都达到极大值亚暴的膨胀相是由行星际磁场转向或南向分量变小触发的，其起始位置很可能位于近地等离子体片内边缘。
Furthermore , competition assay was used to detect the ability of mutant sedn23a , sedn23a / h26r and sedf45a binding to mhcii . the tcrv ? specificity of these mutants was then determined with facs . the results are as follows : ( t ) we confirmed that f45 of sed was an important residue binding to mhc ii 检测了sed突变体促人和小鼠t淋巴细胞的增殖活性，筛选出促增殖活性降低的突变体；进而用竞争结合实验检测突变体与mhc11的结合活性，并用流式细胞仪检测突变体tcrvp特异性变化。
3 immunogenicity of m3m4 loop recombinant peptide antigen of nr1 , neuroexcitotoxicity protective effects and their mechanisms study of antiserum against m3m4 ( 1 ) to explore the immunogenicity of m3m4 loop recombinant peptide vaccine , m3m4 recombinant peptide was injected subcutaneously to immunize balb / c mice ( h - 2 ) , cd4 / cd8 t lymphocytes subsets , the cytokine levels in serum and the liter of specific humoral response were detected with facs , indirect elisa and elisa separately ，抗血清还可以结合合成肤nps ，证明该表位具有免疫原性和抗原性。收集抗血清用于功能检测。 (二) m3m4片段抗血清抗兴奋毒性损害作用以台盼蓝染色法和原位末端标记( tun ’ el )法观察抗血清对原代培养海马神经元兴奋毒性坏死和凋亡的保护效应。
