The eg cell culture media consisting of dmem medium supplemented with fbs , chicken serum , beta - mercaptoethanol , l - glutamine . hepes , chicken embryonic extract and cytokines etc . after 24 hours culture , the isolated pgcs were selectively attached on the gonadal stromall cells in the plates 加入新鲜的eg细胞培养液(含dmem 、胎牛血清、鸡血清、 -巯基乙醇、 l -谷氨酰胺、 hepes 、鸡胚浸出液以及细胞因子等成分)培养24小时以后， pgcs开始部分贴附于共培养的生殖原基细胞上。
Aes contains a glutamine - rich ( q ) domain in its n - terminal , which sharing high homology with the amino terminal region of groucho / tle ( transducin like enhancer of split ) . the function of aes is not clear yet , while groucho / tle were found to be a family of transcription co - repressor and play impo rtant roles in notch signaling , wnt - 0 catenin signaling and other transcription regulation events Aes的n -末端约130个氨基酸中富含谷氨酰眩( q )残基，形成一个q结构域，与转录协同抑制分子tle ( transducinlikeenhancerofsplit )的氨基端高度同源。
The feeding of glutamic acid , glutamine and a - keto - glutaric acid respectively showed that glutamic acid and glutamine had no obvious effects on cell growth , but stimulated apramycin production greatly . with the same concentration of amino acids supplemented , glutamine showed a stronger stimulation effect than glutamic acid , while a - keto - glutaric acid showed a repression effect on apramycin production . it could be deduced from above results that glutamine possibly is the donor of nitrogen element for the biosynthesis of apramycin Glu 、 gln及?酮戊二酸添加实验结果表明glu 、 gln对菌体的生长无明显的影响，但能强烈促进安普霉素的生物合成，且相同浓度条件下gln的促进作用明显高于glu ，而?酮戊二酸则对安普霉素的生物合成有抑制作用。
