Recent researches implicate that raplgap may interact with different type of alpha subunit in heterotrimeric g protein and suggest a cellular cross - talk between small g proteins and heterotrimeric g protein 最近研究发现，某些g亚单位可与rap1gap结合而调节rap1gap的活性或影响异源三聚体g蛋白的信号转导。
In order to study the upstream and downstream components in g protein and calmodulin signal pathways , a yeast two - hybrid system was used to screen interaction partners of these two proteins 本文利用酵母双杂交系统分别筛选了与这两种蛋白相互作用的蛋白质，以进一步研究g蛋白和cam信号转导途径中的其它上下游组分。
Surprisingly , in both of archaebacteria and chloroplast , ftsz is the main component in the process of division but it is n ' t effective in mitochodria division , instead is a dynamin protein 此外ftsz还是一种400个残基的保守蛋白， ftsz与真核tubulin在结构、功能和进化上有一定的相似性及同源性，都具gtpase结构域，属于g蛋白家族。
Synthesis of heparin - bsa complex used a chemistry method , established an elisa technique to detect the interaction of heparin with protein in vitro . elisa is a simple , effective method to study the interaction of polysaccharides and proteins 将hbc (相当于0 . 5拜g蛋白含量)包被在酶标板上， elisa方法检测肝素与ifn一y的相互作用，用相同蛋白含量的bsa一对照作为对照。
This response was mediated by gi and subsequent activation of phospholipase c ( plc ) , which triggered two pathways : protein kinase c ( pkc ) led to repulsion , and inositol 1 , 4 , 5 - triphosphate ( ip3 ) receptor activation led to attractive turning 信号转导机制的研究表明， sdf - 1和baclofen激活了生长锥表面的g蛋白耦联受体，通过gi将信号传递给plc ，进而激活pkc和ip3信号通路。
Then we cloned the mature ca fragment and the same region of the selected ca fragment from library plasmid to vector pgadt7 , but neither did they interact with ga in the assay of two - hybrid system . then we used arabidopsis cam2 isoform as bait to screen the two - hybrid library 之后将成熟ca和文库中筛到的ca编码部分的基因分别亚克隆至双杂交激活域载体pgadt7 ，进行双杂交检测它们与g蛋白的相互作用，结果仍为阴性。
Hd biosciences co . , ltd has announced an important research collaboration with organon , the human health care business of akzo nobel . the agreement relates to the development by hd biosciences of functional assays for a number of identified g - protein coupled receptors and ion channels 根据这项协议，华大天源将为欧加农公司构建一系列的g蛋白偶联受体家族和离子通道家族的筛选模型，这些筛选模型均适应于高通量药物筛选。
Here we report that growth cones of rat cerebellar axons in culture turned away from a gradient of sdf - 1 , a chemokine that attracts migrating leukocytes and cerebellar granule cells via a g protein - coupled receptor ( gpcr ) 以前的证据表明， sdf - 1 ，一种经典的chemokine ，能够吸引白细胞和迁移中的神经元，这种作用是通过g蛋白耦联受体介导的。利用生长锥转向分析，我们发现sdf - 1的浓度梯度能够吸引或排斥培养的小脑颗粒细胞的生长锥。
We obtained two positive clones after screening . sequencing and blasting results showed that the two clones had the same sequences and encoded part of ked like protein in arabidopsis . the coding region was from n terminal amino acid 240 to c terminus 测序及序列比对结果表明二者序列相同，为ked样蛋白的部分编码序列，编码部分从n端第240位氨宋林夜:利用酵母双杂交系统筛选与g蛋白及cam相互作用的新型蛋白基酸至c末端，共207个氨基酸。
Rap - specific gaps have no sequence homology with rasgap and other gaps such as rangap and rhogap . rap is the only one which does not have the invariant gin ( gin 61 in ras ) in switch ii which introduce the arginine finger on gap into active site of ras to complement catalytic machinery 由于rap1gap和rasgap及其它小分子g蛋白的gap (如rangap 、 rhogap )相比在序列上没有同源性，并且rap是唯一一个在switch上没有保守谷氨酰胺的小分子g蛋白。
