According to marketing segmentation theory and positional theory, the paper considers the corporation ’ s ability and resource and chooses the professional hybridized pig culturist and scattered hybridized pig culturist . after making a market research and gaining the data and using the joint-analytical method of orthogonal design method to calculate the product preference of market culturist, then comparing the market position of competitor, the paper ensures kangda corporation ’ s product position to supply the differential marketing strategy 在此分析基础上,本文根据市场细分和定位理论,结合公司的能力和资源,评估和选择了土杂猪专业户和土杂猪散户这两个具有发展潜力的细分市场,并根据市场调查获得的数据,采用基于正交设计的联合分析法分别获得了目标市场养殖户购买偏好,结合竞争对手的市场定位,确定了公司市场定位,为后续的差异化营销策略制定提供支撑。
Total cellular rna were extracted, 40 micrograms of the total rna were used in the reverse transcription reaction, using superscript ii reverse transcriptase, oligo ( dt ) i8 primers, and cy3-dctp or cy5-dctp for the experimental and control group respectively . the labeled cdnas were hybridized to microarrays at 42c for 12 h-18 h 提取as_2o_3作用k562细胞前后的总rna，用superscript逆转录酶逆转录成cdna第一链，并在逆转录的过程中，用cy3cy5荧光染料分别标记对照组处理组，与自制的k562细胞基因表达谱芯片杂交。
The ph ultra-smal1 biosensor was successfuly used to detect ph value in singie cell . wr biosensors for proten and glucose were also proposed . ped one : in chapter l, three ams were synthsized to find a suitable probe to hybridize the expression of ing1 gene, a recentiy identified tumor suppressor gene 并且，将能表达ing1基因的质粒转入肿瘤细胞进行培养后，再将分子信标转入肿瘤细胞，发现转导了质粒的肿瘤细胞比没转导质粒的肿瘤细胞内的荧光明显增强，其增强程度可用光谱扫描的方式将其量化。
The results showed that nb14 and ny 13 had positive hybridization signals, but no signal in the parent-common wheat, and nb14 fragment only had positive signal hybridized with line24 . therefore, we can not deduced it is a responsive gene at present . much work still needed to confirm it 继续用ny13片段为探针，进行southern杂交分析，结果只与多枝赖草有杂交信号，且信号强，进一步证实该est是多枝赖草总基因组dna本身所具有的特异序列，杂交片段本身很可能是多枝赖草六侧协迫应答新基因之一或其一部分。
To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2-hybridized strains were re-confirmed and selected for this study . the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e . coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e . coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e . coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e . coli ( ehec ) and eaggec probe for entero-aggregative e . coli ( eaggec ) . the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e . coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e . coli o157 : h7 为探讨携带小肠结肠炎耶尔森氏菌的hpi毒力岛的大肠杆菌是否具有其他已知的毒力基因，选取82株由原位杂交和pcr方法初筛irp2阳性的大肠杆菌菌株，进行在致泻性大肠杆菌的25个毒力基因的检测，包括肠产毒性大肠杆菌的热稳定毒素st和热不稳定毒素lt，肠侵袭性大肠杆菌的侵袭蛋白b基因ipab，肠致病性大肠杆菌的eaf、espc基因，肠出血性大肠杆菌的溶血素hly、志贺毒素1(slt1)、志贺毒素2(slt2)基因，肠集聚性大肠杆菌的eaggec探针，以及在泌尿道致病性大肠杆菌和o157：h7大肠杆菌中新发现的毒力岛基因。
Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months . the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa . through three clonings less diversed anti-p24 hybridoma cells are gained 方法：基因工程p24抗原免疫小鼠4次，历时2个月，取脾细胞与骨髓瘤细胞株sp20，用peg融合，hat选择培养和间接elisa筛选分泌抗p24抗体阳性的杂交瘤细胞，三次克隆化后得稳定分泌抗p24抗体的杂交瘤细胞株。
We found that sit variant gene ( slt2vha ) was identified in strains e . coli o157 : h7 isolated from patients and dung beetles 2000 in xuzhou city, jiangsu province . the primers used for stx2 variant analysis are shown in tablel . genomic dna restriction fragments digested by pstiwere sonthern-blotted and hybridized with an stx2-specific dna probe . the probe was prepared fromed a 285-bp pcr productof the strain 882364 stx2 gene obtained by using the specific primer pair ( tablel ) 2000年在江苏省徐州市铜山县腹泻病患者的粪便标本分离的10株产生志贺毒素的菌株以及从蜣螂肠道分离到的4株产生志贺毒素的大肠杆菌o157：h7，属于另两个pfge型，和1986、1987、1988年在徐州市腹泻病患者的粪便标本分离的菌株pfge图谱不同。
Although bt cotton altogether can increase yield by 9.6 %, the yield performance of bt cottons hybridized by monsanto or caas genes acquired from informal channels with local cotton varieties is 5.6 % less than that of caas and monsanto formal seeds, though it's yield is 1.4 % higher than that of non-bt cottons . bt cottonseeds with different quality have different impacts on yield . the high quality cottonseeds can increase cotton yield 14 % more than inferior cottonseeds 虽然bt抗虫棉总体可以提高棉花单产9.6，但是通过非正式渠道获得中国农科院(caas)和孟山都(monsanto)bt基因并与当地品种复交后的不能确定基因来源的抗虫棉种子产量表现比caas和monsanto的正规种子至少要低5.6(虽然比常规的棉花品种还是高1.4)。
The work on physical mapping of the chromosome of s . nanchangensis ns3226 was initiated . nearly a full set of chromosomal asei-bamhi fragments of s . nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library . thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion, suggesting distinct identifications 此外，还开展了南昌链霉菌ns3226染色体物理图谱构建的前期研究工作：基本克隆到了南昌链霉菌ns3226染色体上全套的ase-bamh片段，以它们为探针从南昌链霉菌ns3226的基因文库中钓到164个阳性克隆，并从中筛选到34个aselinkingcosmids，用bamh进行初步的酶谱分析，结果表明其中有20个cosmids的bamh酶谱相互间没有明显的重叠性。
The work on physical mapping of the chromosome of s . nanchangensis ns3226 was initiated . nearly a full set of chromosomal asei-bamhi fragments of s . nanchangensis ns3226 were cloned and used as probe to hybridized against its genomic library . thirty four asei linking cosmids were observed from 162 hybridizing cosmids and 20 of them showed no obvious overlapping each other by bamhi digestion, suggesting distinct identifications 此外，还开展了南昌链霉菌ns3226染色体物理图谱构建的前期研究工作：基本克隆到了南昌链霉菌ns3226染色体上全套的ase-bamh片段，以它们为探针从南昌链霉菌ns3226的基因文库中钓到164个阳性克隆，并从中筛选到34个aselinkingcosmids，用bamh进行初步的酶谱分析，结果表明其中有20个cosmids的bamh酶谱相互间没有明显的重叠性。
