To isolate hfl - , hf2 gene encoding flavonoid - 3 ' , 5 ' - hydroxylase ( f3 ' , 5 ' h ) from petunia hybrida of the purple flower , we used a polymerase chain reaction ( pcr ) amplification using the genomic dna as a template . the result indicated that the hfl dna was 2797bp in total length and encoded 506 amino - acid , containing three extron and two intron ; hf2 dna was 2223bp in total length and encoded 509 amino - acid , containing three extron and two intron . comparison of the sequence with genbank databases revealed that hfl dna and hf2 dna shared significant nucleotides identity ( 98 . 97 % and 99 . 04 % respectively ) and amino - acid identity ( 99 . 41 % and 99 . 41 % respectively ) Hf1dna全长2797bp ，包括3个外显子， 2个内含子，其中一个开放阅读框架( orf )编码506个氨基酸，与文献报道的序列相比较，具有98 . 97核苷酸同源性与99 . 41氨基酸同源性： hf2dna全长2223bp ，包括3个外显子， 2个内含子，其中一个开放阅读框架( orf )编码509个氨基酸，与文献报道的核苷酸同源率为99 . 04以及氨基酸同源率为99 . 41 。
In the present study , in order to investigate the effects of endogenous estrogen on the daergic terminals in amy and the daergic neurons in midbrain , fast cyclic voltammetry ( fcv ) was used to examine da release evoked by electrical stimulation from amy of female rats in different phases of estrus cycle , ovx rats and male rats . and tyrosine hydroxylase ( th ) immunohistochemi stry was employed to measure the numbers of daergic neurons of ventral tegmental area ( vta ) and substantia nigra ( snc ) in the rats 因此，为进一步探讨大鼠内源性雌激素水平的变化对amyda能神经系统及中脑da能神经元的影响，本工作应用快速周期伏安法( fcv )在体监测了处于动情周期各期雌鼠、 ovx鼠和雄鼠经电刺激诱发的amyda释放，并应用酪氨酸羟化酶( th )免疫组化方法测定了以上各组大鼠腹侧背盖区( vta ) 、黑质( snc )的th阳性神经元数目。
After i . c . v . 6 - ohda , the c - fos expression of these areas induced by cold stress reduced significantly . 2 ) double staining showed that fos - like immunoreactive positive granules were observed in some vasopressin ( avp ) - immunoreactive positive neurons in paraventricular and supraoptic nuclei , as well as in some tyrosine hydroxylase ( th ) - immunoreactive positive neurons in nts and lc nuclei ( 2 )双重染色显示，寒冷应激诱导的fos样免疫反应阳性颗粒可见于室旁核( pvn )和视上核( son )中的部分加压素( avp )阳性神经元及孤束核( nts ) 、蓝斑( lc )中的部分酪氨酸羟化酶( th )阳性神经元。
