instar

• The bmn cells and fifth instars were infected by the recombinant virus bacpak - angiostatin . the bioactivity of the protein product was determined by human umbilical vein endothelial cells ( ecv304 ) growth assay in vitro and inhibition of vascular growth assay in cam in vivo . angiostatin showed significantly inhibitory effect on endothelial cells
  重组病毒以moi = 10感染家蚕细胞( 2 10 ~ 6个细胞/ 5ml )和家蚕5龄幼虫，表达产物用体外培养的人脐静脉血管内皮细胞( ecv304 )及体内鸡胚尿囊膜( cam )新生血管实验检测其抑制活性： (一)血管抑素可明显抑制体外培养的内皮细胞增殖。
• Suggesting that the larvae of spodoptera exigua died more quickly when fb - 28 were added to the virus . the larvae of spodoptera exigua inoculated as newly molted fourth instars ( 40s ) and at various hours after molting ( 316s ) , we sacrificed larvae at various time intervals after inoculation to process the larvae for lacz expression in midgut by using tissue section techniques on early phase infection
  此外，对于处于不同虫龄的幼虫进行病毒感染时发现：对3 ~ ( 16 )幼虫毒力生物测定时，荧光增白剂的增效作用达到50 ，但对4龄初的供试幼虫进行测定，荧光增白剂的增效作用却不很明显，仅为10 。
• These results suggested that the 65kda protein was a degraded product , bioassay result showed that insecticidal activity of bl - 3 was 3 - fold higher than that of bl - 2 against 3rd instar plutelh xylostella . 3 . expression of cryla ( b ) gene in pseudom on a s fluoreseent pfx - 18 in order to study expression characteristics of icps gene in pseudom onas fluorescent , a new simple transformation method of pf was established
  3 .用上述重组技术获得了含有刀口基因的lz20和含有尸2拼即“的lz20a克隆株，通过基因的诱导表达，发现p ”和pz浏民有不同的作用机制，证明了p20具有保护cyt人溶细胞的作用，这种作用并不依赖大肠杆菌受休的不同而改变
• Transgenic bt cotton mainly affect high - instar larvae by retarding the growth and intering the proceeding of ecdysis , pupation , and eclosion . the growth retardation leads to the postponement of growth period duration ( the showed that growth period duration prolonged 17 days ) . feeding the larvae with the tissue of the transgenic bt cotton and the moths with the flower of transgenic bt cotton , the number of eggs and hatching rate decreased obverviously
  转基因药防田中，天敌昆亚群落的个体数占整个昆虫群落总个体数的10 . 0 % ，其优势种为蜘蛛类、七星瓢虫( coceinellas叩te即uneitatalinnaeus ) 、龟纹瓢虫( prol ， laea少只ponjcagoeze ) 、大眼蝉长蜷( prqp . ylaeajaponicagoeze )和草岭(助rys叩asepre即unetatawesmael ) 。
• It is believed that the search behavior of the third instar larva of e . balteatus de geer is extensive search before feeding , and is area - concentrated search after feeding . 8 . contacting with aphid , eating an aphid and contacting with agar block with aphid body fluid on it can arouse searching behavior of e . balteatus de geer larva from extensive search to area - concentrated search , and contacting with agar block can not
  8 .明确了和蚜虫接触、完全取食1头蚜虫及和沾有蚜虫体液的琼脂块接触等食物刺激均可激发黑带食蚜蝇幼虫搜索行为由广域型转换为地域集中型，而和琼脂块接触则不能刺激其搜索行为转换。
• The morphological diagnostic characters for many mature and early instar larvae are still lacking in china . partial sequences of the mitochondrial cytochrome oxydase i and ii and a transfor rna ( co i co ii and trna ) gene of the adults and larvae from caddisflies were . lepidostomaflavum , l . fui , l . arcuatum , paraphlegopteryx morsei , apsilochorema unculatum and apsilochorema hwangi , and the larval and adult stages of these species were sequenced and associated
  采用dnastarpackage中的editseq软件进行序列编辑、 orf查找；采用clustalx软件进行序列比对( alignment ) ；比对结果输入mega2 . 1软件计算各样品间的遗传距离，并基于kjmura2 - parameter模型，用邻接法( neighbor - jojning ， nj )构建系统发生树，通过自展( bootstrap1000次)检验获得系统树分支的置信度。
• When every s . guan carried about 1 10 ^ 7 spores of coccoid beauveria bassiana , and touched the 4 5 - instar of m . alternatus larva with 1 : 1 , 1 : 2 and 1 : 3 , the ratios of m . alternatus amounts to s . guan amounts , the parastizing infecting death rates were 55 % , 75 % , and 100 % respectively ; s . guan carried coccoid beauveria bassiana to control m . alternatus in the wood section , and the rate was 87 . 13 %
  每只管氏肿腿蜂携带球孢白僵菌约1 10 ^ 7个孢子时，对4 5龄松墨天牛幼虫按虫蜂比1 : 1 、 1 : 2和1 : 3接蜂，寄生感染死亡率分别为55 % 、 75 %和100 % ；管氏肿腿蜂携带球孢白僵菌防治木段内的松墨天牛幼虫寄生感染死亡率为87 . 13 % 。
• Fusion gene by pcr was inserted into bombyx mori baculovirus transfer vector pbacpak . 8 and contransfected with lineared dna of bm - bacpak6 virus into bmn cells . the homologous recombination occurred inside the cells , and the recombinant virus bacpak - 6aa - hgm - csf was expressed , as identified by pcr and southern hybridization . the bmn cells and the fifth instars were infected by the recombinant virus bacpak - 6aa - hgm - csf
  本研究首先通过pcr将家蚕杆状病毒多角体蛋白起始密码子后的18个碱基引入到hgm - csf基因的5 ’端之前，然后将融合基因重组与家蚕杆状病毒转移载体pbacpak8中，获得重组转移载体pbacpak8 - 6aa - hgm - csf ，并与线性化bm - bacpak6dna共转染家蚕细胞株，获得重组病毒bacpak - 6aa - hgm - csf 。
• Several researchers reported that chironomid larvae is difficult to inactivate using free chlorine at concentrations commonly used in drinking water utilities . in addition , the 1st instar larvae can easily penetrate sand filter and then goes into waterworks reservoir and municipal service pipe due to its motility
  1龄摇蚊幼虫具有较强的游动性,可以穿透滤池进入到清水池中,由于常规的水处理消毒工艺对其难以有效地灭活,在我国一些大中城市的管网水中都曾发现过摇蚊幼虫。
• Nutrient and some chemical substance of teleogryllus derelictus gorochov have been measured from 1 instars , 3 instars , 5 instars , 7 instars , 9 instars , adult of 3 days after emergence and adult of 10 days after emergence . the result as follows : the average content of protein of the different growth stages of teleogryllus derelictus gorochov amounts to 15 . 92 %
  试验根据黄褐油葫芦的个体发育过程，选取卵、 1龄、 3龄、 5龄、 7龄、 9龄若虫、羽化3日的雌、雄成虫和羽化10日的雌、雄成虫10个发育阶段，分别测定其虫体所含的营养成分和化学物质。