After selecting kanamycin resistant transformants on ms medium , their dna were extracted for pcr and dot blot . in pcr , the 1 . 488kb fragment was amplified , and the result of dot blot was positive 生长曲线表明，带有克隆片段的转化株在0 . 5mol lnacl的高渗透压培养基中生长良好，而作为对照的缺失株则几乎不能生长。
The optimum concentration of kanamycin was different at different stage of culture , which was 50mg / l at calli induction stage , 100mg / l at embryogenic calli selecting stage , and 50mg / l at plantlet root elongating stage , respectively 把愈伤组织诱导阶段的kan浓度定为somg / l ;愈伤组织继代的kan浓度定为100m叭;小植株长根的培养基的kan浓度定为som叭。
Whether the gene was transformed into the arabidopsis was confirmed by pcr - southern blotting , southern blotting and northern blotting . 1 . pcr - southern blotting showed that all kanamycin - tolerant plants had strong positive signals , and no signal was shown in wild type plants Pcr结果扩增出1 . 4kb的特异性条带，表明sod2已整合进拟南芥中， pcr - southern进一步证实了pcr结果的正确性。
There was no evident difference between the cultures of variety tx0014 and anti - pseudomonas 3 for their sensitivity to kanamycin ( kan ) . the leaf discs turned yellow and calli were hardly induced at 50mg / l kan concentration in the medium m1 番茄tx0014和抗青枯3两个品种对kan的敏感程度差异不大，当kan浓度为50mg l时，叶盘黄化，几乎不形成愈伤：而50mg / l的kan使番茄植株不能生根和生长。
