Laminin a 5 chian is widly expressed in the adult tissue , blieved to be the mainly a chian of mammal . g domain is the most important domain of a chain . in this experiment , e3 ( lg4 - 5 ) , lg4 and lg5 was cloned from the mouse kidney through rt - pcr 鼠的层粘蛋白的5链在成年组织中广泛表达，被认为是鼠体内的主要的链， 5链上的g结构域是其重要的功能域，层粘蛋白的许多的生物功能与其密切相关。
The aim of this study was to evaluate the differences among oral vh , vc and scc by silver - binding nuclear organizer regions stain ( agnors ) and immunohistochemical staining for proliferating cell nuclear antigen ( pcna ) , type iv collagen and laminin 本研究目的希望藉由核仁组成区嗜银蛋白染色，以及增殖细胞核抗原、第四型胶原蛋白及板层素之免疫组织化学染色，做为疣状上皮增生、疣状癌与鳞状细胞癌之区别。
In light of the above results , we suggest that a combination of agnors and immunohistochemical staining of pcna , type iv collagen , and laminin might be used as a method for distinguishing the differences among oral vh , vc and scc 由以上的结果，我们认为合并使用核仁组成区嗜银蛋白染色及增殖细胞核抗原、第四型胶原蛋白及板层素之免疫组织化学染色，可做为口腔之疣状上皮增生、疣状癌与鳞状细胞癌?别诊断上的参考。
Laminin is not only the mainly structural molecule of extracellular matrix , but also the important signal molecule . laminin and collagen iv compose the net - structure of the extracellular matrix , given the tissue of elastic , tenacity , water - holding power , amortization of mechanical power , ect 层粘蛋白与型胶原蛋白构成网状的细胞外基质结构，赋予组织以韧性、弹性、保水性及对机械力的缓冲性，并为细胞提供生存及从事活动的微环境。
Levels of fasting blood glucose and 24h urinary microcontent of albumin 24 h malb were determined dynamically ; the serum glycosyl hemoglobin hba1c was determined after the last medication ; the ultrastructural changes of kidney were observed by transmission electron microscope ; the expressions of collagen , fibronctin , laminin ln , and the ecm metabolism influencing factors , including mmp2 , tissue inhibitor of metalloproteinase timp2 , transfer growth factor 1 tgf 1 in renal tissue were detected by immunohistological chemistry and image collecting analytical system 动态检测各组大鼠空腹血糖fbg 24h尿微量白蛋白24h malb ，末次给药后测定大鼠血浆糖化血红蛋白hba1c透射电镜观察各组大鼠肾脏超微结构改变，应用免疫组化技术及图像采集分析系统测定各组大鼠肾脏组织中型胶原c纤维连接蛋白fn层粘连蛋白ln的表达，测定影响ecm代谢的基质金属蛋白酶2 mmp2基质金属蛋白酶抑制剂2 timp2及转化生长因子1 tgf 1的表达。
