To reasonably plan out the scheme for the sewage discharge outlets of changshu fluorine chemical industry project , the article makes an ana lysis on the water environmental features of changshu section of the yangtze riv e , develops a plan two - dimensional model of coupled water flow - quality for its co mplicated underwater topography and stream flow - field . the model is relatively ac curate and practicable to predict the impact of scheme on water quality . varied s chemes have been compared and demonstrated from multiple angles , such as flow fie ld , impact assessment of water quality , technique and economy . the scheme of sewage discharge outlets away from the shore is recommended . the article provides a sci entific basis for the engineering design and envirommental management of the pro ject 为合理确定常熟氟化工项目排污口布置方案，本文在分析长江常热段水环境特征的基础上，针对其复杂的水下地形和水流流场，建立了较为准确和实用的平面二维水流水质耦合数学模型，预测计算了三套方案污水对重点保护水域影响程度和范围，并从水流流场、水质影响评价、技术经济多角度对方案进行了比较论证，最终推荐污水江中排放的方案，为项目的工程设计和环境管理提供了科学依据。
Finally , in order to investigate whether these two virus are resistant to complement attack , and whether the resistance were mediated by incorporating cellular complement regulators of cd55 , cd59 into their out membrane , complement lysis assay and plaque reduction assay were carried out respectively to find the lysis or neutralization activity of complement to hiv and eev under the condition of with or without the cd55 and cd59 blocking antibody 将这两种病毒分别暴露于人血清补体后，发现在有cd55 、 cd59中和性抗体存在的情况下，补体溶解hiv病毒的效率明显增加； eev病毒的感染活性在空斑实验中也明显降低，结果与这两种蛋白在病毒表面（ eev ）存在的事实相符，提示病毒表面的cd55
Activation of the complement may lead to killing of the microorganisms by direct lysis effect of complement system or the phagocytosis by the enhancement of the attachment of microbes to the phagocyte ( opsonization ) . low serun concentrations of mbl in man is the basis for a common opsonic defect associated with recurrent infection 它可选择性识别多种病原体表面的糖结构，以不依赖抗体和c1q的方式激活补体，发挥溶破和间接调理功能，还能与吞噬细胞胶凝素受体结合而起直接调理作用。
After the recombinant plasmid pcdna3 . 1 / ts87 was identified by digestion of hindlll and bamh i , it transformed into cos7 by lipofectamine . expression product was identified by immunohistochemical method , sds - page and western - blot . the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3 . 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas . l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t . s artificially and sera from rabbit immunized with soluble antigen of t . s and with protein expressed by ts87 gene and by a monoclonal antibody of t . s 通过细胞的免疫组化，细胞裂解物的sds - page电泳， westem - blot分析检测目的基因的表达情况。免疫组化结果显示：重组质粒转染的细胞质中有棕褐色颗粒，而空载体转染细胞及正常细胞无此现象；细胞裂解物sds - page电泳结果显示：只有重组质粒转染的细胞在约38kd处有明显的蛋白带，这与理论计算的ts87基因表达蛋白的分子量为38kd基本一致； western - blot分析结果显示：约38kd的蛋白带能够分别被旋毛虫感染兔血清，成虫虫体可溶性抗原免疫兔血清， ts87基因原核表达蛋白免疫兔血清( ts87血清)以及一株具保护性的旋毛虫单抗特异识别。
So some methods suitable to large plasmid extraction , including lysis by sds and a method from a literature , were used to try to extract the large plasmid from the strain cell . the lysis reactions in these two methods are gentle , so the large plasmid cannot be injured in the lysis process , opposite to lysis by alkali . it would be helpful to keep the integrality of the large plasmid during the extraction 因此我们采用了适合于大质粒提取的sds法，和文献中应用于硝基苯降解性质粒的提取方法，来尝试对菌株细胞进行质粒提取，这两种方法裂解反应温和，不会像碱裂解法那样，在裂解过程中损坏质粒，可以实现质粒提取的完整性。
Firstly on the basis of describing the basic theory of teaching material , this paper analyzes the compiling principles , guidance ideas according as relevant theory of evaluation of teaching material compiling . afterwards it commences on static analysis of the text of the new teaching material from the following four aspects : frame construction , content layout , format layout and compiling features , which indicates that the new teaching material is characteristic of the modernization , exploration and readability as well as the trend of humanism , localization and informationization . then according to the statistic of investigating feedback data from students and teachers , it focuses on dynamic ana lysis of the practical operating position of the new teaching material 在论述教材基本理念的基础上，本文首先根据教材编写的评价理论分析了新教材的编写原则、编写指导思想；接着对新教材的文本从框架结构、内容编排、形式编排、编写特色四个方面进行了静态分析，表明新教材具有时代性、探究性、趣味性、人文化、本土化、信息化等特色；然后根据对学生和教师调查反馈资料的统计，对新教材的实际使用情况进行了动态分析。
High quality tea with natural herbs that has obvious effect to your stomach , and be used widely , obviously lyses the discomfort , feel eased by various kinds of gastric disease for the people who have no disease drink it can prevent then form suffering gastric disease and improve their health gradually . taste good , no bitter and as stringent of drug , drink it just like a perfect tea . has no side - effect even drink for a long time 因此，对于生活节奏紧张，饮食不规律，或嗜酒吸烟因而常觉腹部不适的人们来说，定时饮一杯中国保健茶胃宁茶，不仅能使您沉浸于乌茶的清香中暂时远离尘世的喧嚣，同时更能您的胃肠感到前所未有的舒畅，远离胃部病痛的烦恼。
As an important innate immune system , and as an important arm of the humoral immune response , the complement system is immediately ready to target and eliminate virus particles , to lysis those virions that have lipoprotein membranes , or to prevent it from entering host cells , or to marker them for destruction by other branch of the immune response . at the same time , the host normal tissue are protected from damaging by complement through recognizing the regulators of complement activation ( rca ) expressed on self cells 作为机体重要的天然免疫防御系统及特异性体液免疫应答的重要效应系统，补体系统除了具有溶解、清除病毒等致病微生物，阻止病毒进入靶细胞，调理病毒的吞噬等重要功能外，还可通过“识别”自身组织细胞表面的补体活化调节蛋白来对自身细胞加以保护，使之不受侵害。
The expression protein products as insoluble inclusion bodies accounted for 15 % and 30 % of whole bacterial protein . two methods , b - per reagent only and triton - urine with sonication lysis of cells , were both access to the relatively good washing effect 通过摸索iptg诱导浓度，诱导温度，菌体收获时间等条件，确定了eo基因能在pgex4t bl21 ( de3 ) codonplus和pmal - p2x bl21 ( de3 ) codonplus中高效表达，表达产量分别占菌体总蛋白的15和30 ，表达的重组蛋白主要为包涵体。
