As explants , were infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring the expression vectors pbgsb and pbglsb respectively and co - cultured for 3 days in darkness on the culture medium ( ms + 0 . 1 mg / l 6 - ba + o . l mg / l naa + 0 . 7 % agor ph 5 . 7 ) Ling inaa ）中暗培养三天后，移入加入抗生素100mg lkan和500mg几cr的诱芽培养基，于组培室中进行诱芽和抗性筛选。
Cotyledon and true leaves of stem mustard were cultured on ms medium supplemented with different concentrations of various cytokinins ( ba , cppu , tdz or kt ) alone or in combination with naa or iba , and the adventitious buds regeneration rate was evaluated . 以榨菜子叶、真叶为外植体进行离体培养，用ba 、 cppu 、 tdz和kt等细胞分裂素和生长素naa 、 iba组合诱导子叶、真叶再生不定芽试验。
Anti - keratin auto antibody ( ak auto ab ) is accepted generally as a significant component of natural auto antibody ( naa ) which is found in human as well as vertebrate , and is of characteristic of polyreactivity , low avidity , extensive connectivity and shorting of specificity to the species 天然自身抗体( naa )广泛存在于人和脊椎动物体内，具有多反应性、亲和力低、缔和性广及种属特异性差的特点。
This paper deals mainly with the extraction of geological and hydrogeological information from deep strata through the comparative analysis of major elements , minor elements and trace elements in rocks and in relics of dried fountains by means of neutron activation analysis ( naa ) 摘要利用中子活化测试结果，对岩石及泉水乾涸残馀物中常量及微量元素的进行分析对比，以?解深层岩体有关的地质与水文地质信息。
Is one of the most valuable and most widely cultivated small fruits in the world . while the global soil salt stress and drought caused more and more serious affection on strawberry production . it is necessary to culture salt and drought resistant strawberry cultivars via foreign gene transformation 以不同品种草莓(达赛和丰香)花药为外植体，在附加6 - ba与2 ， 4 - d 、 6 - ba与naa的ms培养基上进行培养，均可诱导出愈伤组织。
Calli were induced by culturing hypocotyls of pepper in the ms medium supplemented with 2 . 0mg / l 6 - ba and l . omg / l naa . the diploid wound callus was treated by adding colchicines into the medium and by immersing into colchicines solution for different concentrations and periods 通过将不同浓度的秋水仙碱加入培养基和不同浓度的秋水仙碱溶液浸泡两种方法处理愈伤组织，从而筛选出最佳的诱导方法和最佳的秋水仙碱浓度和处理时间。
( 3 ) regenerating plantlets were obtained via somatic embryo , which derived from hairy root . the effects on the induction of somatic embryos from hairy root were investigated using orthogonal test . the best somatic embryo induction was obtained on ms + 0 . 5mg / l naa + 0 . 5mg / l kt + 30g / l sucrose 单独使用naa很难诱导出体胚，单独使用kt或kt与naa配合使用均能诱导出体胚， naa 、 kt和蔗糖对体胚诱导的影响作用依次是t a蔗糖。
But cluster buds can not be obtained from the callus of stems and embryo . it has likely relate to the inner structure of callus . lt is very difficult to get embryoid from mature embryo and its inducing rate is very low , and only 11 . 5 % in the media containing n6 + ba 1 . 5mg / l + naa 0 . 5mg / l + sucrose 30g / l + agar 8g / l + ac 2g / l which is the best media for gingo tissue culture 而茎段和胚诱导的愈伤组织基本上没有丛生芽发生，这可能和不同愈伤组织的内部结构有关。成熟胚的胚状体诱导率较低，诱导相当困难，在n6 bai
Bap performed more important function than kt in differentiation of tall fescue embryogenic calli , but better results could be achieved with combination of 2mg / l bap and 0 . 5mg / l kt . at this cytokinin level , 0 . 5mg / l naa was recommended to obtain the highest callus regeneration frequency . plant regeneration could be evidently boosted when embryogenic calli were pre - differentiated on high - osmoticum medium with 60g / l sucrose , and / or when the pre - differentiated compact calli were differentiated on differentiation medium solidified with l0g / l agar 高羊茅胚性愈伤组织分化时， bap的作用要比kt大，但2mg lbap与0 . 5mg lkt配合可获得更佳的效果；在该细胞分裂素水平下，生长素naa用0 . 5mg l ，愈伤组织再生率最高；胚性愈伤组织先在含60g l蔗糖的分化培养基上高渗预分化，以及经高渗预分化后的致密愈伤组织在琼脂浓度为10g l的分化培养基上分化，能明显促进愈伤组织的植株再生；在分化培养基中添加脯氨酸导致愈伤组织再生率下降，但同时有减少白化苗再生的趋势。
S . regeneration system of mustard hypocotyls and cotyledons of 4 days and leaves of 25 days were used as transformation materials , and ms as basic medium supplying different ba ^ naa and kt concentration , we got the optimum explant adventitious bud inducing medium ( ms + 6 - ba3 . 0mg / l + naa0 . 2 mg / l ) . among the three kinds of mustard explants . the cotyledons showed the highest adventitious bud regeneration capacity . and the regeneration capacity of leaf explantswas weaker . and the weakest of hypocotyls 芥菜高频再生体系的建立以4天左右的叶用和茎用芥菜子叶、下胚轴和25天苗龄的芥菜无菌苗的叶片为外植体，以ms为基本培养基，通过加入不同浓度和不同组合的ba 、 naa 、 kt ，筛选到了外植体不定芽分化的最适培养基( ms + ba3 . 0mg / l + naa0 . 1mg / l )及生根的最适培养基( ms + naa0 . 2mg / l ) 。
