Thus , it will help elucidate the development , self tolerance of self autoreactive b lymphocyte repetoire to investigate the generation of naa . researches on the function of naa will help clarify the significance of naa ' s existence . the exploration of biological nature , encoded gene and the difference or relation between naa and pathological autoantibody will shed much light on the pathological mechanism and treament of autoimmune disease 因此，对naa产生机理的揭示将有助于回答免疫学b细胞产生、发育以及自身耐受的问题；对naa功能的研究有助于进一步明确naa对机体的意义；对naa的特性、编码基因、与病理性自身抗体界限和关系的研究可能对自身第四军医大学硕士学位论文免疫疾病的发病机制和治疗提供有益的线索。
It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions , clearing aged cells and metabolic products , regulating immune responses and protecting against infection . in some pathological states such as psoriasis and contact dermatitis , a certain serum level of the antibody could inhibit the progression of the diseases , and is beneficial to the recovery from the diseases . after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies , meanwhile , experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) " , a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability , immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis , and recombinant antibody is under investigating 抗角蛋白自身抗体( akautoab )是naa的重要组成部分，以往实验通过杂交瘤技术、免疫亲和层析技术和噬菌体抗体库技术分别获得单克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，并对akautoab免疫学特性及在体生理和病理意义进行了广泛的研究，直接或间接地发现akautoab是机体正常免疫调节网络的组成部分，在维护某些生理状态的稳定、清理衰老细胞及代谢产物、调节免疫和抗感染等方面起到重要作用；在某些病理情况下(如银屑病、接触性皮炎等) ，体内akautoab的组分和滴度会发生变化，而正常水平的akautoab则有利于限制病情的发展，促进损伤的修复。
No callus occurred from hairy roots on medium lacking 2 , 4 - d . high concentration ( l . 0mg / 1 ) of 2 , 4 - d could induce much more calli , but it obstructed the growth of calli and followed buds differentiation . the result of callus induction showed that the optimum medium for callus induction was ms + 0 . 5mg / l 2 , 4 - d + 0 . 5mg / l 6 - ba and ms + 0 愈伤组织诱导最佳培养基为： ms 05mg l个d 05mg l ba ，愈伤组织生长的最佳培养基为ms 0ling 12 ， 4 d cling 16ea ；愈伤组织能在不含激素的培养基上自发地分化出不定芽，但分化频率较低，而6ea对不定芽的分化作用较明显，配合使用6习a和naa对芽的诱导及得芽率较好，不定芽诱导的最佳培养基为ms 10mgilnaa 05mg lia培养基。
The regeneration system of tobacco was established . the adventitious shoots were induced from leaf explants of tobacco based on ms basal medium supple - - mented with 2 . 0mg / l 6 - ba and 0 . 3mg / l naa . then the regenerated plants were rooted on ms medium containing 0 . 3mg / l naa 植株再生体系的建立采用无菌苗烟草叶片为外植体，以ms为基本培养基，筛选出ms + 6 - ba2 . 0mg l + naa0 . 3mg l以诱导不定芽的分化，并于ms + naa0 . 3mg l的pa硕士学位论文v沉了砂“工队nk ”主根培养基上生根，获得完整再生植株。
