Plasmid pbbbast - pinii has the potato proteinase inhibitor ii ( pin ii ) gene which encodes potato proteinase inhibitor . the sip gene was digested by restriction enzyme hindlll and inserted into the vector pbbbast - pin ii 质粒pbbbast - pin含有马铃薯蛋白酶抑制剂基因pin ( potatoproteinaseinhibitor ) ， pin基因编码的蛋白酶抑制剂具有广谱抗虫性。
To allow secretion of the crylac protein into the intercellular space , potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac 运用pcr技术克隆了马铃薯蛋白酶抑制剂基因的信号肽序列，并将其分别连到crylac 、 gfp基因的5 ’端，构建植物转化载体p3301ubisigac和p3301ubisiggfp 。
The result showed that organo - inorgano - mixed fertilizer could increase the quantity of availability , strengthen the activity of proteinase , sucrase , urase and peroxidase in the sugarcane roots , and increase the sugarcane yield and sugar content 结果表明，有机无机肥料配施，可提高土壤有效养分的含量，激活土壤蔗糖酶、脲酶、蛋白酶、过氧化物酶的活性，促进甘蔗生长与蔗糖分的累积，对甘蔗增产增糖有积极的作用。
The main factors including foam - positive proteins and yeast proteinase a that influence foam stability of unpasteurized beer and the mechanism of these proteins affecting the foam are introduced , then the strategy for foam improvement are indicated in this paper 摘要简要介绍了纯生啤酒泡沫稳定性的主要影响因素泡沫阳性蛋白和酵母蛋白酶a对啤酒泡沫的影响及影响机理，并简要介绍了改善纯生啤酒泡沫的主要策略。
However , by far , all the milk - derived antihypertensive peptides ( also known as angiotensin converting enzyme inhibitory peptides , aceips ) are produced by the proteinase hydrolysis of milk proteins , no report is available on aceips expressed by dna recombinant technology 然而，到目前为止，所有的乳源抗高血压小肽都是通过蛋白酶对乳蛋白质的水解获得的，还没有通过基因工程技术来表达抗高血压小肽的研究报道。
2 ) the method of proteinase k isolation and the method of ctab isolation had been compared carefully while extracting the genome dna from crickets . the result showed the method of proteinase k isolation of total dna was more suitable for the extraction of total dna from insects of grylloidea 2 ）在提取蟋蟀总科昆虫的基因组dna时，比较了ctab法和蛋白酶k法两种方法，结果显示蛋白酶法更适用于蟋蟀总科昆虫的基因组则a的提取。
Introduced a variety of textile products with biological agents and enzyme products , amylase , cellulose enzyme , proteinase , acid cellulose enzyme , neutral membrane proteins hydrolysis enzyme , but also complex hemei , heat , temperature , and low - temperature enzyme , naisuanjian proteins 产品介绍各种不同的纺织用生物酶及其制剂产品，有淀粉酶、纤维素酶、蛋白酶、酸性纤维素酶、中性纤维素酶等水解酶，也有杂合酶，高温、中温、低温酶、耐酸碱酶等。
Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k . a 109bp fragment was amplified from the human genome dna . 2 . in 166 han samples , 19 ( 11 . 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp 用红细胞裂解液和pcr缓冲液非离于去污剂蛋白酶k两种自配试剂成功地提取了所收标本的人白细胞基因组dna ，并以此为模板成功扩增出预期的109hpmbl目的基因片段。
In this study , a new approach of plant virus - resistance was expored by making up the cdna of ppiv . nib gene was amplified from plasmid pysr , which is plant expression vector containing potyvirus y nib gene . the nib gene was fused with the fragment encoding the mature part of papaya proteinase iv 将番木瓜蛋白酶的cdna序列以全长、去除信号肽部分和成熟酶部分分别连接到细菌表达载体中，表达结果表明，只有含去除信号肽部分cdna的载体才能检测到相应的表达的蛋白条带。
There are at least three kinds of ep in petal : thiol - proteinase , metallo - proteinase , serine proteinase . they gather at the site between 43 kd and 66 . 2 kd ( about 52 kd ) on the substrate gel electrophoresis . the optimum ph of thiol - proteinase , metallo - proteinase is ph 6 , which are looked as acid ep , the serine proteinase has two forms , one is acid ep ( ph 6 ) , and the other is alkali ( ph 10 ) 花瓣中都至少有3种内肽酶：巯基蛋白酶、金属蛋白酶、丝氨酸蛋白酶，三者聚集在底物胶电泳胶条的43kd 66 . 2kd之间的位置，约为52kd ；前两种最适ph为6 ，是酸性内肽酶，后一种有酸( ph6 ) 、碱( ph10 )两种存在形式，既是酸性内肽酶又是碱性内肽酶。
