Zhoukou bangjie leather produce company is one of subsidiaries of henan bangjie group limited liability company located in henan zhoukou city , which was honored as " leather city " with rich goad leather and scalper leather . 邦杰皮革制品公司河南邦杰集团皮革制品公司是河南邦杰实业集团有限公司下属子公司，是中国皮革工业协会理事单位，河南皮革工业协会常务理事单位。
However ， cracking down on scalpers needs every passenger ' s cooperation : in order to guarantee your interests are not violated ， please book train tickets by phone and your tickets will be delivered soon to your doorsteps or go to the sales windows to buy tickets in person 然而，打击票贩子需要每位旅客的帮助：为确保您的利益不受侵害，请电话订票，享受送票上门或者亲自到售票窗口买票。
There are no establishment of qinchuan - scalper skin cell lines until 2002 . the present experiment aimed to establish qinchuan - scalper skin cell lines . the culture method and characteristics of qinchuan - scalper skin fibroblast and epithelial cells were studied 本试验应用细胞培养技术，研究了秦川牛皮肤组织细胞的分离及原代培养方法；首次建立了秦川牛皮肤上皮细胞和成纤维细胞系；并对成纤维细胞的生长特性进行了深入探讨，结果表明： 1
There are two goad leather , scalper leather production line , products includes goad leather for suit , shoes , glove , scalper leather for shoes etc . more than 50 varieties . all leather resource is purchased in local area , and all chemicals materials is introduced from german , holland , french , america . our products is popular in beijing , shanghai , zhejiang , jiangsu , hebei , henan , shandong , and guangdong for its choice materials , 与港商合资在广州建有后道涂饰分厂，年生产山羊皮60万张，黄牛皮10万张，主要产品有：牛羊兰温皮牛羊坯革羊皮擦色革抓花革转印革打腊革珠光革仿鹿革油变革水晶鼓软革纳帕软面革等十三大类50多个品种。
4 ) either low - sugar dmem , tcm199 , dmem + f12 ( 1 : 1 ) support qinchuan - scalper skin fibroblast cells growth well , but low - sugar dmem caused best effect 5 ) eof and igf - i added in low - sugar dmem could promote qinchuan - scalper skin fibroblast cells growth potent respectively , yet they have cooperated effect each other 在低糖dmem中添加egf和igf _ ( - 1 )均可以对秦川牛皮肤成纤维细胞增殖能力的提高有显著效果，其中igf _ ( - 1 )促增殖作用稍强于egf ，且二者具有协同作用。
6 ) after passage , the qinchuan - scalper skin fibroblast cells were in lag phase in first two day , then entered log phase and persisted six days , they stayed at plateur phase for about three days ; the mitotic index were most high at the fifth day after culture and toboggan at the sixth day 从生长曲线可以看出，以低糖dmem培养第4代秦川牛皮肤成纤维细胞，接种后滞留期缩短，约一天左右，之后为对数生长期，持续六天左右，进入平顶期。分裂指数最高时期在培养开始后的第五天，第六天急剧下降。
The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell , collegenase i ( 150iu ml - 1 " ) and trypsin ( 0 . 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue , which is appropriate to obtain qinchuan - scalper skin cells 组织块法、分散单细胞及dispase冷消化法单细胞均能获得好的秦川牛皮肤组织原代培养物。其中150iu ? ml ~ ( - 1 )胶原酶i与0 . 05胰蛋白酶( 1 ： 1 )同时应用能更好的分离秦川牛皮肤组织，是获得秦川牛皮肤细胞的适宜方法。
