Therefore , the 4 . 4kb fragment from pbl - 2 was ligated to a shuttle vector pht304 with bt replicon and transformed into acrystalliferous mutant 4d10 ( serotype hm , ) and resulted in a cloned strain bl - 3 . this strain could express only 130kda protein 它不仅对苏云金芽胞杆菌工程菌的构建具有现实意义，而且对其它基因高表达研究将产生积极影响，具有一定的理论意义和潜在的应用价值
For patients fully recovered from dengue fever , is a second infection possible ? there are four serotypes of dengue virus . patients fully recovered from one particular serotype may contract the disease again if bitten by mosquitoes carrying a different serotype of dengue virus 登革热可由四种不同类型的登革热病毒所引致，曾感染其中一种病毒的康复者，如再被带有其他类型登革热病毒的病媒蚊叮咬，便有机会再次染病。
In this study , we transformed the vp1 gene of fmdv serotype o into the tobacco chloroplast and chlamydomonas reinhardtii chloroplast , and achieved highly expression levels , paving the way for engineering an fmdv vaccine via transplastomic plants 在本文中作者将o型口蹄疫病毒vp1抗原基因分别重组和转化到模式植物烟草和衣藻的叶绿体基因组中，并使其得到高效表达，为利用植物叶绿体表达系统生产口蹄疫基因工程疫苗提供了技术依据。
Information obtained so far revealed that there have been 62 cases of meningococcal infection in anhui province from december 20 , 2004 to january 30 , 2005 . most of the patients are students and some of the cases are believed to be caused by serotype c 直至目前为止，资料显示由二四年十二月二十日至二五年一月三十日安徽省共出现６ ２宗脑膜炎双球菌感染个案，大部分病者为青少年，而部分个案相信是由丙型脑膜炎双球菌引起。
In addition , laboratory results showed that the local case reported last week involving a 61 - year - old man living in sham shui po was of serotype 2 virus . the man developed fever on september 25 . he was admitted to caritas medical centre on september 29 and discharged today 此外，化验显示上星期发现涉及一名在深水?居住的六十一岁男士的本地个案，属第二型病毒，他在九月二十五日出现发烧的病徵，于九月二十九日入明爱医院，今日已出院。
Once recovered , immunity to that serotype of dengue virus will develop . but second and third infections with other serotypes of virus are more likely to result in severe dhf . the symptoms are severer than those of first infection with a higher fatality rate 第一次感染登革热的病情一般都较轻，愈后对该类型病毒会产生免疫能力，但当第二或第三次感染其他类型的病毒时，出现严重的登革出血热的机会较高，病情会比第一次严重，而死亡率亦会较高。
Once recovered , immunity to that serotype of dengue virus will develop . but second and third infections with other serotypes of virus are more likely to result in severe dhf . the symptoms are severer than those of first infection with a higher fatality rate 第一次感染登革热的病情一般都较轻，愈后对该类型病毒会产生免疫能力，但当第二或第三次感染其他类型的病毒时，出现严重的登革出血热的机会较高，病情会比第一次严重，而死亡率亦会较高。
The chickens and chicken embryos were inoculated with variant serotype isolate e of infectious bursal disease virus using cloacal , nosal routes or via the allantoic cavity route , and the histopathological features of the bursa of fabricius of the ibdv _ infected chickens at various intervals of time were systematically investigated 本试验全面而系统地观察了传染性法氏囊病病毒变异e株，通过泄殖腔、鼻腔和尿囊腔接种雏鸡和鸡胚后不同时间法氏囊的组织形态学变化。
" farmers should report foot - and - mouth disease cases to the afcd as soon as possible . it is in their best interests to do so as this allows the afcd to provide farmers with veterinary diagnostic services . it also enables us to monitor the serotype of the virus so that an appropriate vaccine could be chosen if a new serotype is found , " afcd s assistant director ( inspection and quarantine ) mr lai ching - wai said 渔护署助理署长（检验及检疫）黎清惠说：猪农应尽快将口蹄病个案通知本署，向署方举报是符合猪农的最佳利益，以便我们为其牲畜提供诊症化验服务，以及监察病毒的血清型，如发现新的血清型，便可选择适合的疫苗。
However , the sequence indicated no homology that of cryla ( b ) and cryla ( a ) . sds - page showed that el - 1 and el - 2 expressed full - length 130kda protein in e . coll however , the recombinant plasmid from el - 1 and el - 2 , was transformed respectively by electi operation into acrystalliferous mutant bti ips 78 / ll ( serotype h14 ) and resulted in bl - i and bl - 2 2 .将p了夕和cy时基因以及它们重组休插人大肠杆菌表达载休puhe一24 ，分别获得了3种重组质粒plz19 、 plzcyta和休21ga .将重组质粒转化到不同大肠杆菌受休菌xli ， dhs仪，获得了5种含有不同基因型的转化子
