To establish a simple , inexpensive , rapid technology to detect point mutation or snp of mbl gene with high sensitivity and specificity , which should be of utility in the most of medical laboratories in our country 建立敏感性高、特异性强、简单快捷、适合我国国情(条件较差)的mbl基因点突变和snp的检测技术。
The biological significance of this snp needs to be investigated . a set of monoclone antibodies fmu1 - 7 against cd226 have been made in our lab , immunolized by natural cd226 protein 另外，我室用天然分子制备了一套cd226的单克隆抗体fmuj ， fmui 5识别的是cd226分子胞膜外区domain ， fmu6和fmu7识别domain2 。
Establishing snupe for 2 y - snp loci , we understand that the suitable thermostable dn a polymerase and proportion of template and primer are the keys to reaction of snupe 通过建立2个y - snp的snupe反应，了解snupe反应的一般规律，揭示单碱基引物延伸反应的关键是选择合适的dna聚合酶及合适的引物和模板的比例。
The synthesized single strain oligonucleotides were separated by high performance liquid chromatography . the thermostable dna polymerase was used to catalyze snupe and the reactions of snupe for two y - snp loci were developed 利用耐热dna聚合酶催化单碱基引物延伸反应，建立2个y - snp的单碱基引物延伸反应。
The rule of snupe was revealed for analyzing y - snp of forensic science . methods a total of 8 novel y - str loci were retrieved from database of internet . primers of dys443 and dys444 were redesigned by us 方法在数据库中寻找到8个新的y - str基因座；利用软件重新设计其中的两个基因座，即dys443和dys444的引物。
To quantify the products of single nucleotide primer extension ( snupe ) , the single strain oligonucleotides were separated by high performance liquid chromatography ( hplc ) . the reactions of snupe were established for two y - snp loci 建立单碱基引物延伸反应，掌握单碱基引物延伸反应的一般规律，为法医学y - snp分析提供基础。
With strong specificity and easy to manipulation , this simple , inexpensive , rapid molecular beacon hybridization technique permits visual monitoring of gene point mutation and snp , which shows better advantage than pcr - sscp . 2 Mb杂交技术特异性强，简单快捷，能进行可视性检测，完全适用于点突变和snp的研究，技术特点明显优于pcr sscp 。
We confirmed one single nucleotide polymorphism ( snp ) site in the exon 7 of cd226 gene with agt - ggt polymorphism of 307 codon . among ten volunteers , 4 cases are serine and 6 cases are glycine at this codon 在编码cd226胞浆区第307位氨基酸的密码子上存在agt ? ggt单核昔酸多态性（ singlenucleotidepolymorphism ， snp ） ；分别编码丝氨酸和甘氨酸。
Objective in order to improve the discrimination power and exclusion chance of polymorphic markers on chromosome y , it is necessary to cast about for novel y - str loci and to develop a new method to genotype y - snp for forensic science 目的为了提高y染色体遗传标记的个人识别能力和非父排除能力，寻找新的y - str和建立新一代遗传标记y - snp的检测方法十分必要。
The cumulus expansion , cumulus cells dna fragmentation , oocyte meiotic maturation and degeneration were determined 44 h after incubation . snp inhibited cumulus expansion and cumulus cells dna fragmentation in a dose - dependent manner 结果发现， snp可剂量依赖性地抑制卵丘细胞的扩展和dna片段化，同时抑制ceos中卵母细胞的减数分裂恢复，抑制m向m期转变。
