In the ventral hippocampus , median and intralaminar nuclei of the thalamus and interpeduncular nuclei , nkr - li was localized in neuropil . colocalization of nig - li and vp were studied in paraventricular nucleus and superoptic nuclei . the excited nk3 - containing neurons expressed fos in major part neurons of paraventricular nucleus 在室旁核和视上核双标神经元占nk3第四军医大学博士学位论文阳性神经元总数的65和42 ，占加压素阳性神经元总数的60和39 。
But how about the morphological feature and the relationship between the pv - li and other neurons in the second - / third - order relay nuclei . what the synaptic connection between the pv - li and the neurons projecting to the thalamus . it is well known that glutamate ( glu ) is a major excitatory neurotransmitter in mammalian cns 但是二、第四军医大学硕士学位论文三级中继核团内pv样阳性神经元的形态学特征及pv样阳性结构与其它神经元、纤维和终末的联系究竟如何，它们与向丘脑投射的神经元形成怎样的突触联系都是值得探讨的问题
Injected group , 0 . 1 % saccharin ( 1 . 5 ~ 2ml / rat , in 5min ) intraoral infused group and cta group . the expression of endogenous leucin - enkephalin ( lek ) in the rat brain was observed and 5 parts of the thalamus including laterodorsal thalamic nucleus ( ld ) , lateral part of mediodorsal thalamic nucleus ( mdl ) , ventroposterolateral thalamic nucleus ( vpl ) , ventroposteromedial thalamic nucleus ( vpm ) and reticular thalamic nucleus ( rt ) were comparatively researched before and after the acquisition of cta applying lek - immunocytochemistry . in behavioral experiment , 18 adult male sd rats were divided into normal cta group ( control ) and 2 naloxone i . p 为探讨cta形成过程中enk的作用，本实验用成年雄性sd大鼠35只，分为空白对照组、生理盐水( 2体重)腹腔注射组、 0 . 15mlicl溶液( 2体重)腹腔注射组、 0 . 1糖精溶液口腔灌流组( 1 . 5 - 2ml只， 5min )和cta建立组，采用免疫细胞化学方法，观察了亮脑啡肽( lek )阳性神经元在大鼠脑内的分布情况，并比较了各组大鼠丘脑外侧背核( ld ) 、丘脑内侧背核外侧部( mdl ) 、丘脑腹后外侧核( vpl ) 、丘脑腹后内侧核( vpm )以及丘脑网状核( rt )等5个脑区内lek表达水平的差异；另外将成年雄性sd大鼠18只，分为正常cta建立组以及在cta建立前或cta建立后阿片受体拮抗剂纳洛酮( 2mg kg体重)腹腔注射组，对内源性阿片样物质对于cta建立和保持的影响进行了行为学研究。
