We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously . there are three kinds of combination probability that could be observed by gfp marker 在实验中同时共转染人集缩素smc亚基hcap一e和hcap一c特异的rnai质粒rhe和rhc和pcdna3 . 1 + kg到hela细胞。
This report explored the function of the human smc subunits - hcap - e and hcap - c using rnai and transfection techniques . we constructed two rnai plasmids - rhe and rhc that specifically corresponded to hcap - e and hcap - c of human condensin , respectively 为此我们构建了对人集缩素中两个smc亚基hcap - e和hcap - c特异的rnai质粒rhe和rhc 。
The optimal expressing time with the calcium phosphate method was 72h after transfection , and the transfection method of calcium phosphate is proved to be more effective than the lipofection method 结果表明，采硕士论文戴君勇： k亚科动物的亲缘关系及hsblys的真核表达用cos 7作为真核表达细胞株，其表达目的蛋白的最佳时间是72小时。
In this study , using bioscien tomato as test material and the flanking region of the bioscien insert sequence was determined using inverse pcr and real - time pcr . . by means of verification , this sequence is specific for the transfection event 本研究以华番1号为试材，利用反向pcr和荧光pcr技术已成功将华番1号转基因整合位点dna序列扩增出来。
Direct damage on supf trna gene can be neglected because half - life of mnng is 1 . 1 hour and the interval between treatment and transfection was as long as 12 - 24 hours . therefore the mutagenesis is not lesion directed 20m的mnng经洗涤后的极微量残留经过10 ? 20多个半衰期的降解后，己不足攻击转人的qdna分子，因此这种突变显然是发生在mnng直接攻击部位以外的正常碱基上。
The common inducing protocols , including specific inducers , five - step induction , stromal cell coculture and gene transfection , are not very effective , with the shortcomings of complicated manipulation and high costs 目前常用诱导方法包括使用特异性诱导剂、 “五步诱导法” ，还有尚有争议的基质细胞共培养法，以及基因转染与筛选，但有操作复杂、费用高、毒性大等缺点。
To study the biological function of sh2a gene , we constructed its recombinant expression vec - tor , and investigated its function by cell transfection , kinase assay , subcellular localization and expression analysis . materials and methods 1 我们构建了sh _ 2a基因的真核重组表达载体，通过细胞转染、激酶活性检测、亚细胞定位、表达分析和流式细胞仪等方法对其功能进行了初步的研究。
3 . polyethylenimine ( pei ) , a water - soluble cationic polyelectrolyte , has been extensively used as a deformable and mobile substrate for a biomembrane for it high transfection efficiency of chemical - based delivery systems . what ' s more , pei is good buffer in physiological environment ( ph < 9 ) 3 、聚乙烯亚胺( pei )是一种阳离子型的聚合物，由于它在动植物细胞内优良的转染效率，而被广泛的应用，并且在生理环境下( ph 9 )它具有很强的缓冲能力。
Further , eif - 5a overexpression through transfection promoted up inhibitor - induced apoptosis , and . mutation of eif - 5 a abolished the enhancing effect , we could conclude that the accumulation of hypusine - absent eif - 5a played an important role in the strong induction of apoptosis by up inhibitors 进一步的分析结果表明过表达eif - 5a ，可增大细胞对mg - 132的敏感性，而hypusine突变体无此现象，说明未形成hypusine的eif - 5a可促进阻断泛素通路诱发的凋亡作用。
On the base of previous research , we used human recombinant growth hormone , estrogen , lps to treat hepatoma cells ( bel - 7402 ) to explore the regulation of a - 1bgp expression . we also investigate the possible proliferation function of a - 1bgp by means of pcdna3 . 1 - a - 1bgp transfection into cells ; finally we construct ppiczaa - a - 1 bgp vector , then transform i t into gs115 in order to acquire the secretory recombinant a - 1b glycoprotein . materials 1 本研究是在原有研究的基础上，应用重组人生长激素( gh ) 、雌激素( - e2 )及脂多糖( lps )处理人肝癌细胞，检测对- 1bgp的转录调控作用；通过基因转染的方法，观察- 1bgp对细胞增殖的影响；最后构建ppicz a - - 1bgp表达载体，将其转染入毕式酵母gs115菌株，从而获得分泌的重组人- 1b糖蛋白，为进一步研究其蛋白功能奠定基础。
