Two expression systems were used , one of which was qiaexpress system , the other glutathione s - transferase ( gst ) gene fusion system )系统和谷胱甘肽s ?转移酶( gst )基因融合表达系统。
Transferase an enzyme that catalyzes reactions in which entire groups or radicals are transferred from one molecule to another 转移酶：是一种催化反应物分子的整体结构或基础部分转移到另一个分子的酶。
But the ppl researchers have succeeded in knocking out only one copy of the gene for the enzyme , called alpha 1 ， 3 galactosyl transferase 但是, ppl医疗公司的研究人员只是成功地去除了产生这种叫? 1 , 3半乳糖转移酶的基因的一个副本。
The mechanism ofchloramphenicol toxicityis apparently failure of the newborn to conjugate chloramphenicol withglucuronic aciddue toinadequate activityof hepaticglucuronyl transferase 氯霉素中毒的机制显然是由于新生儿肝脏的葡萄糖醛酸转移酶活性不足，不能使氯霉素与葡萄糖醛酸结合。
The mechanism of chloramphenicol toxicity is apparently failure of the newborn to conjugate chloramphenicol with glucuronic acid due to inadequate activity of hepatic glucuronyl transferase 氯霉素中毒的机制显然是由于新生儿肝脏的葡萄糖醛酸转移酶活性不足，不能使氯霉素与葡萄糖醛酸结合。
Investigations were carried to assess the sublethal effects of the insecticides ( fipronil and triazophos ) and the heavy metals ( cd2 + and pb2 + ) on activities of brain acetylcholinesterase ( ache ) , hepatic glutathione - s - transferases ( gst ) and liver esterases in pseudorosbora parva 处理在24h时的抑制率为79 . 5 ，是三唑磷的近4倍、处理iv的1 . 6倍，但在48h后，处理i 、处理iv和三哩磷的抑制率基本接近。
2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6 . their antibodies " isotypes are tested by goad anti - mouse isotype regent 取驯化好并处于对数生长期的2 - e _ 4 - a和s _ 2 - b细胞，再常规融合和筛选，三次克隆化后得稳定分泌双特异性抗体的杂交-杂交瘤细胞株6株。
An expression vector carrying a fragment encoding the amino - terminal part of an fr - 008 type i pks module , containing a keto - synthase ( ks ) and part of an acetyl - transferase ( at ) domain was constructed for trial expression of the extremely high g + c content ( 76 % ) pks gene in plant 为探索在植物中表达极高g + c含量的pks基因的可能性，构建了携带有编码fr - 008型pks模块氨基端部分的基因的表达质粒，包括一个酮基合酶( ks )和部分酰基转移酶( at )活性结构域。
Among the genes , there were genes directly related to liver regeneration : fetuin , cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase , gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase , ribosomal protein , and related to stress response : haptoglobin , transferrin 这些基因中有和肝再生有直接关系的如：胎球蛋白、组织蛋白酶；和肝脏功能密切相关的如：胞质天冬氨酸转氨酶、谷胱甘肽硫转移酶；与物质能量代谢有关的如： atp合成酶、核糖体蛋白；以及与急相反应有关的如：触珠蛋白、转铁蛋白。
From the view of cellular rheology , we examined the influence of fluid shear stress on the metabolism , morphology , structure and function of endothelia cells in our previous research . it is first time for us to develop the research between shear stress and apoptosis . in this study , we stimulate the human umbilical venous endothelial cells ( huvec ) by lps and apply different levels of fluid shear stress to them by a parallel plate flow chamber , then observe the change of apoptosis of endothelial cells by terminal deoxynucleotidyl transferase mediated dutp nick end labeling ( tunel ) 我们从细胞力学的角度，曾对血管内皮细胞在剪切力作用下的代谢、形态、结构及功能等进行过系统研究，但剪切力与内皮细胞凋亡的相关研究尚属首次，我们希望通过探讨细胞凋亡与剪切力大小及作用时间的关系，为探索剪切力对血管内皮细胞信号转导的影响及作用机理提供有价值的参考数据和实验资料。
