The first two methods are the most commonly used for soybean transformation . the other methods have not been optimized for soybean , and are therefore less efficient and not often used . previously , we isolated ssnhxl ( encoding a vacuolar na + / h + antiporter ) from s . salsa , and further research was done through the overexpression in arabidopsis thaliana , it could help us to elucidate the salt tolerance of halophyte 本实验室成功的从盐地碱蓬中克隆到位于液泡膜上的ndm ”反向转运蛋白基因ssnhx ，转化拟南芥并对该基因的功能进行了分析，发现在拟南芥中过量表凶亥基因可以巳著提；削6物的灿处性。
The result of routine tissue dyeing assay showed symptoms of hyperemia , swell , fatty , vacuolar and granular degeneration in the liver cells of treated carp ; hyperemia , swell and vacuolar degeneration in the renal tubular epith epithelial cell and lymph cell inroad ; hyperemia , swell , hyperplasia and slough in the gill lamella epithelium ; hyperplasia in lymph cell , gill lamella length becomed different and frame foul - up . the influence become severer with the concentration increasing 其破坏程度随着浓度的增加而愈发严重。建议在使用中一定要科学施药，环境水域中乙氰菊酯的浓度以不超过0 . 685mg l为宜，醚菊酯的浓度以不超过0 . 057mg l为宜，氯氰菊酯的浓度以不超过0 . 020 g l为宜。
Furthermore , aba may activate multiple ca2 + flux in stomatal guard cells , triggering vacuolar k + ( rb + ) release . besides these known components , hydrogen peroxide is a ubiquitous second messenger which has been researched in plants . this paper showed aba - induced h2o2 generation in guard cells of vicia faba , and activation of ca2 + channels by h2o2 本实验利用激光共聚焦显微技术、膜片钳技术以及荧光分光光度技术研究了aba诱导蚕豆气孔保卫细胞产生h2o2 ，并可能是由nadph氧化酶催化产生的，同时也证明了h2o2是引起ca2 +内流的一个关键成分。
Additionally , protein bands were found in the range of 62 kd in microsomes from oryza sativa and 45 kd from arabidopsis thaliana - , 2 ) the ipar - like protein was mainly localized in the vacuolar membrane ( tonoplast ) and plasma membrane in the leaf and root tip of arabidopsis thaliana for confirming the biological role of the ip3r - like protein , we investigated the effects of methyl viologen ( stimulating ip3 produce ) and heparin ( a competitive inhibitor of inositol 1 , 4 , 5 - triphophate in animal ) on stomatal movement by epidermal strip bioassay . when the epidermal strips of arabidopsis thaliana were treated with methyl viologen , it stimulates the stomatal closure 拟南芥表皮条用甲基紫精处理，可以引起气孔的关闭，当处理90分钟的时候，气孔的相对开度由100降到58 ，统计学分析气孔开度的变化有显著差异；甲基紫精引起气孔关闭的作用可以被肝素部分抑制，当甲基紫精和肝素同时处理90分钟的时候，气孔的相对开度由100降到92 ，统计学分析气孔开度的变化没有显著差异，说明气孔的相对开度基本上没有变化。
We analyzed their sequence characterizations , genomic structures and transcription levels under salinity stress . the results indicated that ssvp showed highest homology to the vacuolar h + - ppase ( cvp ) gene from chenopodium rubrum and phylogenetic analysis indicated that ss vp and cvp share a cluster , which showed that they might be more similar in evolution . southern blot analysis showed that there was more than one copy of ssvp in the suaeda salsa genome 本实验从400mmol lnacl处理的盐地碱蓬地上部分构建的zap - cdna文库中克隆了编码mapkk的全长cdna ( ssmapkk ， ay093683 )和编码液泡膜h ~ + - ppase的部分cdna ( ssvp ) ，据已知序列设计引物利用pcr方法获得编码盐地碱蓬液泡膜h ~ + - ppase的全长cdna 。
