丝氨酸

• The he was strongly inhibited by sbti and apmsf , and very sensitive to pmsf , lbti , and tlck , while not sensitive to chymostatin , bestatin , leupeptin , tpck , pepstatin , nem and iam . all these results imply that brine shrimp he was most probably a trypsin - type serine protease . the he could be strongly inhibited by edta in a dose - dependent manner , and 50 mmol / l edta exhibited more than 56 . 5 % inhibition
  对孵化酶纯化样品进行生化性质和酶性质分析发现，卤虫孵化酶的最适反应温度约为40 ，最适ph为8 . 5左右；该孵化酶对p - apmsf 、 sbti极为敏感，对pmsf 、 lbti和tlck也非常敏感，但对chymostatin 、 leupeptin 、 pepstatin 、 bestatin 、 tpck 、 nem和iam不敏感，表明该酶极可能是一种属于胰蛋白酶类型的丝氨酸蛋白酶。
• We obtained the full length gene of hbfgf coding sequence with pcr , and adjusted the g + c content according the software dnasisv2 . 5 , and replaced the cys78 and cys96 with serines by site - directed mutagenesis . 2 . sequence result suggested one of the recombinant is correctly synthezied and cloned
  用pcr方法合成了hbfgf编码区全长，其中前20个氨基酸的g + c含量按暨南大学硕士学位论文：大肠杆菌表达重组hbfgf结构和功能优化摘要照计算机软件计算的结果进行了调整，第78和96位上的半眯氨酸被突变为丝氨酸。
• Quorum - sensing systems can be divided into two paradigmatic classes : luxi / luxr - type quorum - sensing systems in gram - negative bacteria which produce acyl - homoserine lactone ( ahl ) autoinducers , and oligopeptide / two - component - type quorum - sensing ciruits in gram - positive bacteria which produce autoinducing peptide ( aip ) autoinducers . in contrast to the two paradigmatic quorum - sensing systems , vibrio harveyi produces and responds to an ahl - type autoinducer termed ai - 1 and a novel signal molecule named ai - 2
  Qs系统可分为两种典型的类型，即革兰氏阴性细菌的luxi luxr型qs系统，革兰氏阳性细菌的寡肽双组分型qs系统，分别以酰基高丝氨酸内酯( acyl - homoserinelactones ， ahl ) 、寡肽( autoinducingpeptide ， aip )为信号分子。
• There are at least three kinds of ep in petal : thiol - proteinase , metallo - proteinase , serine proteinase . they gather at the site between 43 kd and 66 . 2 kd ( about 52 kd ) on the substrate gel electrophoresis . the optimum ph of thiol - proteinase , metallo - proteinase is ph 6 , which are looked as acid ep , the serine proteinase has two forms , one is acid ep ( ph 6 ) , and the other is alkali ( ph 10 )
  花瓣中都至少有3种内肽酶：巯基蛋白酶、金属蛋白酶、丝氨酸蛋白酶，三者聚集在底物胶电泳胶条的43kd 66 . 2kd之间的位置，约为52kd ；前两种最适ph为6 ，是酸性内肽酶，后一种有酸( ph6 ) 、碱( ph10 )两种存在形式，既是酸性内肽酶又是碱性内肽酶。
• All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene . this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )
  所有的样本通过聚合酶链式反应?限制性片段长度多态性方法( pcr - rflp )对ahsg基因的第7个外显子内的sac多态性位点进行基因分型，该多态性位点为238号氨基酸密码子中间的碱基c到g的替换，使苏氨酸( thr ， acc )变为丝氨酸( ser ， agc ) 。
• Studies revealed that p - catenin dissociated from ccc and translocated into free catenin pool in cytosol after it has been phosphorylated at tyrosine or serine residues , and in this situation , the ccc has been disrupted and cell adhesion function disturbed . a large amount of the free p - catenins in the cytosol can be degraded by the tumor suppressor apc , and the remains translocate into nucleus and bind to transcriptional factor tcf / lef in the nucleus and then promote cell proliferation related gene or anti - apoptosis gene transcription
  当-连环蛋白酪氨酸或丝氨酸残基磷酸化后，就与ajs发生解离而游离到细胞浆中，此时细胞的粘附功能也发生障碍，游离到胞浆中的-连环蛋白，一部分被抑癌因子apc降解，一部分则转移到细胞核内，与核内的转录因子tcf lef结合，启动与细胞增殖有关的基因转录。
• Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease . serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography . the t profile , t stability profile , ph profile , substrate specificity and inhibitor were tested
  该酶分子n -端的氨基酸序列为avidtgveashpef ，通过n -端氨基酸序列设计简并引物，提取被毛孢owvt - 1的总rna ， rt - pcr克隆了该酶的成熟肽编码基因( 1000bp ) ，序列分析表明，丝氨酸含量高达12以上。
• Hydrophobicity analysis predicted a 36 - residue hydrophobic signal peptide for secretion in the n - terminus , and no transmembrane region was present , suggesting it might be a type of secretory protein . some generic and atipical n - glycosylation sites were present in clsp , suggesting that the protein might represent a glycoprotein . the clsp protein contained one cub ( clr / cls , an embryonic sea urchin protein uegf , and bone morphogenetic protein i ) domain from 39th to 164th ammo acids , which is known to be involved in protein - protein or protein - substrate interaction , and a trypsin - like serine protease domain positioned from 244th to 483rd amino acids
  Clsp分子具有补体样丝氨酸蛋白酶的多种结构特征，包括36个氨基酸组成的疏水信号肤，一个cub ( clr / cls ， anembryonicseaurehinproteinuegf ， andbonemorphogeneticproteinl )结构域和一个胰酶样丝氨酸蛋白酶结构域( t汀psin一likeserineproteasedomain )和几个保守的糖基化位点等，没有发现有跨膜区的存在。