The plasmid dna was cut and dephosphorylated . the linearized plasmid dna was ligated to the pseudomonas sp . dna fragments and resulting recombinant plasmids were used to transform escherichia coli xll - blue 提取细菌总dna ，用限制性内切酶部分酶切后，回收2一6kbdna片段，与克隆载体puc18连接，转化大肠杆菌，构建了该菌株的基因组文库。
The restriction enzyme hind iii was used to cut the cucumber dna , and the fragment of the obtained dna is suitable to prepare the cucumber dna voltammetric biosensor . thus the sensor possessed excellent analytical characteristics 采用hind限制性内切酶降解所提黄瓜dna ，使之片断大小适合制备dna传感器，显著改善了dna传感器的重现性等性能。
Reconstruction of antisense pcdnas - doc - 1r plasmid after transformation the pmeiss - fl3 - doc - 1r plasmid into e . coli cells , we have extracted the plasmid and cut doc - 1r fragment using xho i to get a 975 bp doc - 1r cdna fragment Pcdna3 - doc - 1r反义重组质粒的构建将pme18s - fl3 - doc - 1r质粒转化后进行质粒提取。用xhoi限制性内切酶从克隆载体上切取975bp的doc - 1rcdna片段。
By comparing restriction maps of plasmids possessed in these clones , 5 clones ( clones 1 , 4 , 5 , 6 , and 8 ) were found to contain the same chitinase gene , while the other three clones ( clones 2 , 3 and 9 ) contain different chitinase genes one another 经底物反应和限制性内切酶图谱分析，确定其中的clone - 1 ， 4 ， 5 ， 6 ， 8含有相同的几丁质酶基因；而clone - 2 ， 3 ， 5 ， 9四株重组菌则含有不同的几丁质酶基因。
Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site , and the igf - i product of pcr contains 230 base pairs . igf - ii contains 219 base pairs . 3 各另外设计一对特异性pcr引物，导入适当限制性内切酶切点，以上述连有目的基因的克隆载体为模板，采用pcr方法扩增基因片段，获得长度约230bp的igf -和219bp的igf -成熟肽基因序列。
An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv . the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined 将包含全基因片段的三个基因克隆以限制性内切酶消化后顺次连接克隆到载体ptz18r上，构建了4个全长基因的分子克隆，分别命名为pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中两个转移到低拷贝载体plg338上，命名为plgd30343 、 plgd70344 。
It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent , genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study , genetic diversity in a . polytricha was higher than that in a . auricula : 4 in this study , a . fuscosuccinea had a higher homology to a . auricula than to a . polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a . auricular and a . fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a . auricula as a probe which hybridized with a . auricula and a . fuscosuccinea except a . polytricha , further confirming the veracity of the results from eric - pcr ; 7 in this study , isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity 本研究中，木耳属2个种的2个菌株在its区域表现出较高的保守性， 4种限制型内切酶的酶切图谱没有显示出多态性；增加内切酶种类及供试菌株数量，有可能获得具有多态性的限制性内切酶酶切图谱； 9本实验中， its区域的真菌特异性引物与真核生物通用引物对于扩增效果无较大差异，扩增片段长度均为650bp左右； 10根据形态学实验、 eric - pcr实验以及southern杂交实验的结果分析，紫木木耳属种质资源的遗传鉴定和遗传多样性评价耳极有可能是毛木耳种的一个变种; n .本研究中所用的gutc法是一种适用于木耳属菌株基因组洲a快速提取的方法; 12 .传统的形态学分类法和现代的分子生物学分类法，两者的关系是相辅相成，互为验证
