Enzyme cutting analysis indicated that they had different enzyme cutting sites and cutting site number 利用常见的限制性内切酶酶切分析表明，二者有不同的酶切位点和切点数。
The deduced amino acid sequence of hau3r protein suggested that propablely it is a kind of endonuclease similar to ea59 根据其核苷酸序列推译的氨基酸序列暗示hau3 ~ r蛋白可能是一种类似于ea59的核酸内切酶。
To screen the library , defferential screening had been employed . sixty - four clones were randomly picked to perform dot blot 随机挑出13个克隆进行限制内切酶分析，结果有10个质粒含有300一600bp的插入片段。
3 . pbv220 - endostatin was transformed into e . coli dh5a . the positive colony was screened and identified by pcr and restriction endonuclease digestion 3 .将重组质粒转化到克隆菌dh5a中，经pcr筛选和限制性内切酶鉴定，得到阳性克隆菌株。
The genome could be digested only by restriction endonucleases hind , accl and xbal , and was resistant to cleavage by a wide range of other restriction endonucleases 病毒的基因组可以被hind 、 acc和xba酶切消化，但不能被其他所使用的限制性内切酶消化。
The orf of the hbrp coding a peptide of 233 aa , using the dnasis v2 . 5 demo analysis its structure and pi 3 . screening 1 ) screening using restriction enzyme Pcr产物和pgex一sx一1融合表达载体分别用bamhi和x五01限制性内切酶双酶切后连接，构建融合表达载体。
The genomic dna of ginkgo extracted by ctab method is light brown in color . it is neither digested with restriction endonucleases nor acted as template for polymerase chain reaction ( pcr ) 常规的ctab法提取的银杏基因组dna呈黄色，不易被内切酶所消化，也不能作为pcr模板。
The restriction map of phzl318 carrying the entire add gene cluster from s . avermitilis nrrl8165 was made . its two subclones phz2104 and phz2105 were introduced into s . lividans mutant zx1 制作了携带完整add基因簇的phz1318的限制内切酶图谱，根据酶谱进行亚克隆得到phz2104和phz2105 。
The point mutation in vp2 residue 297 of canine parvovirus from ser to ala acid was examined , which causes a alteration of the restriction enzyme aiu i site 根据vp2位点297的改变引起限制性内切酶aiu酶切位点的变化，建立pcr依赖性限制性片段长度多态性分析( prfl )方法，检测vp2位点297的变异。
Methods isolates were identified as acinetobacter calcoaceticus by using antibiotic susceptibility test , plasmid profiles , restriction enzyme fingerprinting assay and plasmid elimination method 方法对我院不动杆菌的感染进行调查，采用药敏试验、质粒抽提和限制性核酸内切酶分析法、质粒消除试验。
