And the positive clones were used as the immunogens to immunize mice . both of the serum and phage peptides were add to measure their effects on the growth of huvec ( human umbilical vascular endothelial cell ) 检验这种短肽可否模拟vegf的抗原决定簇诱发机体产生能与vegf结合的抗体，研究小鼠抗血清对huvec (人脐静脉血管内皮细胞)生长的抑制作用以及噬菌体表达的7肽和12肽对huvec生长的抑制作用。
The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest . then the recombinant plasmid was identified by endonuclease analysis , pcr ampliation and dna sequencing . the report showed that the recombinant plasmid had right open reading frame 重组质粒经酶切鉴定， pcr鉴定和测序，结果证实猪肺炎支原体黏附因子p97基因的抗原决定簇r1区定向插入了质粒pet - 32a ( + ) ，且阅读框架正确。
By immunizing rabbits with aa 192 - 326 fragment , polyclonal sera were obtained that were able to recognize e1 proteins expressed in both e . coli and mammalian cells , suggesting that e . coli - derived e2 proteins carried hcv e1 - specific , glycosylation - and - conformation - independent epitopes 利用aa192 - 326免疫家兔，获得了可识别大肠杆菌和哺乳动物细胞表达之e1蛋白的多抗血清，表明大肠杆菌系统表达的e1蛋白携带有hcve1特异的、不依赖于糖化和立体构象的抗原决定簇。
Attachment to host tissue is essential for colonization by most mucosal pathogens , such as mycoplasma hyopneumoniae . according to the reported sequence of p97 gene in strain 168 , a pair of primers was designed and the rl region of p97 gene in strain 168f485 was amplicated by pcr 本研究根据已报道的序列为参考，设计一对带有ecor和hind酶切位点的引物，并通过引物的定点突变，利用pcr方法从猪肺炎支原体168f485株扩增到其黏附因子p97基因的抗原决定簇r1区。
We paid our main attention to cloning tb22kda gene from tartary buckwheat , expressing the structure gene and getting the purified recombinant protein , and these provide foundation for father study on the relationship between structure and function of the allergenic protein tb22kda , the orientation of the corresponding epitope and the exploration of allergenic reaction mechanism 本研究的目的在于分离克隆苦荞中的主要过敏蛋白( tb22kda )基因，并表达纯化出其结构基因编码的蛋白。从而为进一步研究过敏蛋白tb22kda的结构与功能，寻找其中的抗原决定簇，探讨过敏原与其相应抗体相互作用机理提供依据。
Till now , a protein with 22kda in tartary buckwheat ( tb22kda ) has been identified as the major allergen , so further study of the corresponding epitope in virtue of immunological and molecular genetic methods will be helpful in understanding the molecular mechanism of allergenic reaction and finding proper ways of resisting irritability Tb22kda蛋白是苦荞中的主要过敏蛋白，对此蛋白中抗原决定簇免疫学和分子遗传学的深入研究，将有助于了解过敏反应机制并设计抑制荞麦过敏蛋白的表达或使患者脱敏的方法。
