The piezoelectric quartz crystal impedance analysis is a new method to study the quartz crystal resonance . it has been well established as a powerful tool capable of responding in situ to an electrode mass change down to the nanogram level and the change of the solution viscosity and density , and it is also expected to find wider applications in chemistry , life science , surface science , food science , environment monitoring and so on 压电石英晶体阻抗分析法是研究石英晶体谐振的新技术，可获取多维压电信息，从而现场动态监测电极表面低至纳克级的质量及溶液粘密度等响应，可广泛应用于化学、生命科学、表面科学、食品科学、环境检测等领域。
The detailed investigations are as follows : the first part of the focuses on the development of piezoelectric immunosensor which the immobilization of antibodies with protein a and the study . on kinetics of immuno - reaction using piezoelectric quartz crystal , and the second part is primarily directed to the development of piezoelectric immunosensor which the immobilization antibody with self - assembled monolayers ( sams ) 具体内容如下：本文的第一部分着重于用蛋白a固定抗体的压电免疫传感器的研制以及利用压电石英晶体对免疫反应的动力学研究；论文的第二部分着重于采用自组装技术包埋抗体的压电免疫传感器的研究。
Therefore , the determination of seb is very important for food hygienic analysis as well as for clinical analysis . nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology . the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule 其中b型葡萄球菌肠毒素( seb )是一种通常条件下更稳定，毒性最强的毒素，而核酸杂交技术则是分子生物学和基因工程中最常用和最基本的方法之一，因此本论文以该毒素的产毒基因为检测对象，以压电石英晶体为敏感元件，以合成的寡核苷酸探针为识别分子，构建了用于seb基因检测的压电生物传感器。
Piezoelectric sensing technique has been applied to many fields , including analytical chemistry , life science , environmental monitoring and surface sciences because of it ' s small size , high sensitivity , simplicity , low power consumption and broad sensing spectrum . piezoelectric immunosensors possess high selectivity provided by antibodies or antigens and sensitivity response directly to the mass change of proteins . immobilization technologies of biomolecules are of paramount importance in order to preserve their biological activity 压电免疫传感技术利用压电晶体与抗原抗体特异性免疫反应结合的特征，通常在压电石英晶体表面先固定抗原(抗体) ，响应溶液中抗体(抗原)引起的质量、密度和粘度等性质的变化，它兼具有压电质量响应的高灵敏性和生物化学反应的高特异性。
Piezoelectric immunosensing technique incorporates the high sensitivity of piezoelectric quartz crystal microbalance and high specificity of antibody - antigen immunoreaction , presenting some outstanding advantages including desirable simplicity , rapid response , and so on , which make it showing an attractive future of potential applications in the biochemical diagnosis of clinical diseases 压电免疫传感技术结合了压电石英晶体高灵敏性和免疫反应的高特异性,具有测定过程简便、快速等特点,在生物分析中具有广阔的应用前景。
2 . in this thesis , the fractai reaction kinetics analysis was first to use in the study of heterogeneous reactions happened on the surf8ce of piezoelectric sensor the adsorption kinetic modei of antibody and the immunoreaction kinetic modei of antibody binding antigen on the surf8ce of piezoeiectric sensor were set up . when the models were appiied to the experimentai , good fitting results were obtained 本文首次利用分形反应动力学理论来研究压电生物传感器表面发生的不均一反应并对生物大分子抗体吸附到传感器敏感膜表面，以及发生在敏感膜表面的抗原抗体反应进行初步研究；提出了在压电石英晶体表面发生的大分子吸附反应或抗原抗体反应的动力学反应模型；对实验进行了应用，获得了较好的拟合结果，验证了模型。
Piezoelectric quartz crystal ( pqc ) sensor has found its extensive application in various fields including analytical chemisny , electrochemisny , fore science , life . . science , environmental monitoring and medica analysis because of its outstandng advantaes such as high sensitivity , simple construction , convenient operation and low cost , etc . 压电石英晶体( pqc )传感器作为一种响应谱广、灵敏度高、结构简单、操作方便、成本低廉的传感器，在分析化学、电化学、生命科学、表面科学、膜科学、环境科学和药物科学等诸多领域获得了广泛应用。
