Two of them were rapgap according to dna sequences . to confirm this finding , we re - transformed these two dna fragments into y190 with rapgap individually again . after - lth and lac z check , the conclusion is that two molecules of rapgap protein from c . elegans really interacted with each other in y2h system 将这两个基因片段重新与ppc97rapgap共转化入y190酵母菌，再次经一lth和lacz筛选，并以不同对照组做参照以排除假阳性可能性，结果证明两个分子celopns来源的ra灿ap蛋白在酵母双杂交系统中确实可以相互作用。
By yeast two - hybrid assay , aes was found to interact with gp130 intracellular region through its conserved q domain . results from the yeast two - hybrid assay , gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain , and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction . to investigate the consequence of this interaction , tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector 在通过酵母双杂交分析确定aes通过q结构域与sp130分子胞浆区结合的基础上，为确定tle1分子是否也能通过保守的q结构域与gp130分子胞浆区结合，我们通过pcr扩增编码gp130胞浆区与tleq分子不同结构域的cdna ，构建了含有这些不同结构域的酵母双杂交载体，通过酵母双杂交分析证实： tle1分子通过其氨基端的q结构域与gp130分子胞浆区近膜段结合。
In our work , different hbv pres gene fragments were amplified by pcr , cloned into pgbkt ? vector in yeast two - hybrid system 3 , and then transformed ah 109 yeast cells . the results showed that these pres expression products were nontoxic to yeast cells , but all self - activated the yeast two - hybrid system 本研究将hbv前s区不同片断进行扩增，克隆入酵母双杂交系统3中的pgbkt7载体，转化酵母细胞ah109 ,经检测，证实了hbv前s区不同片断对酵母细胞无毒性作用，但是都存在自激活作用。
In order to assess the function of kyot2 , northern blot , immunohistochemical sabc and in situ hybridization methods were used to find out the expression of kyot and kyot knockout mice which were kept in our laboratory were analyzed . moreover , we employed a yeast two - hybrid screening using kyot2 as a bait protein and got 13 interacting proteins . the function of a novel protein of them , kbp was investigated 为了进一步研究kyot的功能，我们利用northern印迹、兔疫组织化学等方法对kyot在体内的分布进行时空定位，并利用本室懈的kyot基困剔除小鼠分析其表型，同时采用酵母双杂交筛到tkyoth相互作用的13种分子，并对其中的一个新分子kbp进行了功能的研究
