The soluble fusion proteins cbd - lt 27 in cytoplasm and periplasm of e . coli were purified with cbind 100 resin , the purity of fusion protein was more than 80 % in eluted solution 采用cbind100resin从破菌上清中快速纯化可溶的融合蛋白cbd - l7a27 ，洗脱下来的融合蛋白纯度可达80以上。
However , the mechanism of action of angiostatin on endothelial cells is less clear . further structural and functional studies are hampered by a lack of soluble , correctly folded , biologically active protein in sufficient auantity 然而， angiostatin对内皮细胞的作用机制并不十分清楚，其结构和功能的研究往往受无法获得足够量的，可溶的，能正确折叠的，有活性的蛋白的限制。
Raw materials muscle tissue of amino acids , peptides , dissolved in broth , to increase fresh ; the resilience of the collagen in the connective tissue protein in the long heated complete hydrolysis into soluble gelatin , conducive to digestion ; bone tissue of calcium and vd , the reaction of organic acids , which will help absorb : fat group weaving in and cooking fatty acids can occur in the reaction of the alcohol esters , which will help increase incense 原料肌肉组织中的氨基酸、多肽等溶解于汤汁中,利于增鲜;结缔组织中坚韧的胶原蛋白质在长时间加热后完全水解成可溶的明胶,利于消化;骨骼组织中的钙质与vd 、有机酸类发生反应,利于吸收:脂肪组织中的脂肪酸则可以与料酒中的乙醇发生反应生成酯类物质,利于增香。
The results of lauryl sodium sulfate - polyacrylamide gel electrophoreses ( sds - page ) of the aggregate precipitate and supernatant and the result of high - performance size - exclusion chromatography of the supernatant indicated that , by wrongly linked intermolecular disulfide bonds soluble bi - molecular and tri - molecular egg white lysozyme aggregate could be simultaneously formed except being renatured to native and active egg white lysozymes during the refolding procedure of denatured - reduced egg white lysozyme ; the aggregate precipitate could be further formed by the non - covalent bonds interaction between the soluble hi - molecular egg white lysozyme aggregates , and the soluble tri - molecular egg white lysozyme aggregate could still stay at the supernatant 沉淀和上清液的不连续十二烷基硫酸钠聚丙烯酰胺凝胶电泳( sds - page )和高效凝胶排阻层析分析结果表明，还原脲变性蛋白溶菌酶在稀释复性过程中除了能够复性成天然态蛋白溶菌酶分子外，还会形成可溶的蛋白溶菌酶分子二聚体和三聚体，二聚体和三聚体主要是靠分子间二硫键的错配连接而成的；可溶的蛋白溶菌酶分子二聚体之间通过非共价键相互作用而形成集聚体沉淀，而可溶的三聚体溶菌酶分子则仍处于复性液上清液中。
