培养液

• The content of ammonium nitrogen in the medium reduced rapidly during the lag phase , but showed a slightly increase during the rapid - growth phase
  而在生长迟滞期内，培养液中氨态氮的消耗高达35 . 29 g ml ? d ，但进入快速生长期后，氨态氮含量先明显回升，然后又逐渐下降。
• The numhers of day - 4 ebs per milliliter culture media are 44 . 83 1 . 22 ( n = 6 ) and 43 . 17 1 . 05 ( n = 6 ) respectively when cultured by hyclone fbs and homemade green season fbs
  在含进口hyclone胎牛血清或国产四季青胎牛血清的培养液中培养， es d3细胞形成的4debs数量分另为44
• Abstract objective to explore the effect of conditioned medium of various concentrations of human embryonic neural stem cells on the growth of cells in the cultured spinal cord tissues in vitro
  摘要目的探讨不同浓度神经干细胞条件培养液对体外培养脊髓组织中不同细胞成分生长的影响。
• Meanwhile , oxygen concentration of culture medium containing embryos decreased with time ( p < 0 . 0001 ) . with the development of embryos , oxygen exhausting rate trended to increase
  未受精卵耗氧极低，而胚胎培养液中氧的浓度直线下降( p 0 . 0001 ) ，而且随着胚胎的发育耗氧率有上升的趋势。
• The morphological change of the primary hepatocytes in different medium was observed under an inverted phase contrast microscopy . results : hepatocytes adhesion was examined at 12 hr of culture
  利用倒置相差显微镜来观察并摄像记录短期培养两种不同的培养液中的小鼠原代肝细胞的形态学的变化。
• Conclusion the effect of conditioned medium of human embryonic neural stem cells can improve the growth of the spinal cord neurons and neurogliocytes cultured in vitro according to its concentration
  结论人胚胎神经干细胞条件培养液对体外培养的脊髓神经元或胶质细胞促生长作用的差异与其浓度有关。
• After that we replaced the media with fresh complete media every two or three days . for each passage the cells were plated at about 3 x 10 / ml and grew to confluency
  细胞达80 。 t融合后用0 ? 25胰蛋白酶37消化2 3分钟，用全培养液终止消化后， 1000rpm离心5分钟，弃上清后用全培养液重新悬浮细胞。
• To initiate induction , undifferentiated escs were dissociated and replanted on none - coated tissue culture plastic dishes in nsc medium . the medium was changed ever - y other day into serum - free nsc medium
  诱导分化时去饲养层细胞，重新接种于无包被的培养皿中，使用nsc培养液，逐渐更换为无血清nsc培养液。