The cells were harvested and total e . coli proteins were detected by sds - page to select expressed strains . western - blot analysis of the gel that showed a band of similar size was the major protein immunoreactive with the anti - 6xhis mab Rpoifn经部分纯化并充分复性后，用细胞病变抑制法测定rpoifn的抗滤泡性口炎病毒( vsv )活性。
Then formation of three disulfide binds of hw - ma and rgd - hw was monitored by reversed - phase hplc using air oxidization or reduced and oxidized glutathione respectively . the synthetic peptide was purified by double reversed - phase hplc 用hplc分离纯化，得到的合成产物经质谱鉴定， hw一ma和rgd一hw粗肤的实际测定值和复性后突变体理论值都一致，证明合成和氧化复性是成功的。
After dissolving inclusion bodies , renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ) , the fusion protein trx - scfv of electrophoretic purity was obtained , they still possessed antigen - binding activity 经变性复性，用固相金属离子亲和色谱法一步分离纯化了具有功能的单链抗体，在一升的发酵液中得到78mg的重组蛋白。
The research of the renaturation and biological activity of the chimeras confirmed the active sites of hwtx - i and mviia further . it also indicates that the structure of ick is stable and is an ideal model molecule for protein engineering 通过对嵌合体复性和活性情况的研究，进一步证实了hwrtx一i和mvlla的活性位点;并且表明抑制剂胧氨酸结模体( ick )的结构确实是相当稳定的，是一种进行蛋白质工程的较理想的模型
After renaturation , the biological activity of mviia is nearly 100 % of that of natural one ; the activity of hm - 1227 is less than t | iat of hwtx - i ; however , hm - xw cannot block the neuromuscul ar transmission in an isolated mouse phrenic nerve - diaphragm preparation 复性后的mv a摘要生物学活性接近天然mvlla的100 % ;嵌合体hm一1227的活性小于hwtx一i的15 % ;而嵌合体fd以一xw不能阻断小鼠隔神经一隔肌的接头传递。
The accompanying condensed consolidated financial statements do not include any adjustments related to the recoverability or classification of asset - carrying amounts or the amounts and classifications of liabilities that may result should the company be unable to continue as a going concern 附带的简明财务报表并不包括任何有关以下内容调整：可复性或资产结存价值分类或可能导致公司不能持续经营的债务数额和分类。
Another chimera rgd - hw did not showed activity of platelet adhesion and aggregation that rgd sequence is thought to possess after complete refolding and purification , which explained that the resign and the progress of refolding of chimera rgd - hw was required improvement further 另外一个突变体rgd一卜铆经完全复性及分离纯化后没有显示出rgd序列应具有的抗血小板聚集活性，说明这个突变体分子设计和氧化复性过程还有待进一步优化。
The mutants were transfected into pc 12 cells respectively to probed the ability respondence to gdnf stimulation and the interaction with ret by immunoprecipitation , c - ret phosphorylation and immunoblotting assays . the main results are as follows : 1 . expression and purification of recombinant rat gfral protein to obtain recombinant gfral and study its biological activity , the cdna encoding the mature rat gfral was isolated using rt - pcr with total rna extracted from newborn s 纯化和复性后的重组gdnf蛋白，可显著增强pc12一gfral一ret工程细胞的存活和分化;对pc12一ret工程细胞没有任何作用;对pc12一gfral的存活和分化作用11中英文摘要显著强于对pc12一ret的作用，但也显著低于对pc12一gfral一ret细胞的作用。
Gfral was high expressed in e . coli after iptg induction . by ni2 + chelation affinity chromatography , the purified recombinant gfral protein were obtained . based on the evolutionary trace method , multiple sequence alignment and dendrogram construction were then carried out by the clustalx program and constructed a phylogenetic tree from a multiple sequence alignment for a protein family 2 . gdnf的表达及其对pc12基因工程细胞的影响用本教研室已构建好的表达质粒pet一gdnf转化大肠杆菌bl21 ( de3 ) ，经lmmipto诱导gdnf表达，并在niz气nta柱上进行纯化，稀释复性后，纯度达90 %以上。
Corneal surfaces in eyes with regular astigmatism are thought to be toroidal surfaces . based on mathematical models for corneal surfaces before and after treatment , a corneal ablation model is given for such refractive errors as spherical myopia , compound myopic astigmatism , and simple myopic astigmatism . a similar corneal ablation model is also given for such refractive errors as hyperopia , compound hyperopic astigmatism , and simple hyperopic astigmatism 采用超环面( toroidalsurface )建立起规则散光下的角膜曲面模型方程;定量研究了手术光学区屈光不正两种通用的矫正模型,一种模型适用于单纯近视、复性近视散光、单纯近视散光的矫正,另一种模型适用于单纯远视、复性远视散光、单纯远视散光的矫正;另外,作为影响手术效果的重要因素,提出了一种用于光学区直径和过渡区宽度均可调节时过渡区角膜切削量的计算模型。
