The tpsl gene was introguced into immature embryo cells of 12 - 16 days wheat cultivars wen . 6 via gene gun delivery technique . resistant calli were selected on medium containing hygromin ( 40 - 60mg / l ) 用基因枪将海藻糖- 6 -磷酸合成酶基因( tps1 )导入小麦栽培品种温6的12 - 16日龄的幼胚细胞中。
Genotype mainly influences the frequency of embryogenic calli formation , 81 . 35 % immature embryo of yangmai 87158 can form embryogenic calli , while only 16 . 81 % immature embryo of annong 92484 can form embryogenic calli 基因型的影响主要影响到胚性愈伤组织的发生频率，以幼胚为外植体杨麦87158可以达到sl
However , when immature embryo was used as explant , the optimize concentration of 2 , 4 - d is different , as for yangmai 87158 and ann 98005 , it is 2 . 0 mg / l , but as for ann92484 , it is 4 . 0mg / l Omg l时出愈率和胚性愈伤组织诱导率达到最大值，而对于幼胚不同品种表现有所差异，其中杨麦87158和安农98005在2 ， 4浓度为2
In this experiment , the immature embryos were used as explant to induce calli and bt gene was tranfered into mo17 , 81162 , ji 846 and 7922 by the agrobactrium - mediated . we have gained the positive plants determined by pcr 本论文以东北地区骨干玉米自交系幼胚为外植体，采用农杆菌介导法将bt基因导入了mo17 、吉846 、 81162及7922 ，并获得了pcr检测呈阳性的再生植株。
Four green plants were regenerated form resistant calli of wen . 6 derived form 1500 implanted embryos . no green plant was regenerated form calli of 200 non - transformed embryos . pcr assays of 4 green plants showed that two of them attained the expected size of amplified dna fragment ( 1500bp ) 在含有20 - 60mg / l潮霉素的ms培养基上经诱导和继代培养后，获得了一批抗性愈伤组织，经过分化培养获得4株再生苗，对照的200枚幼胚未获得再生苗，对再生苗进行pcr检测。
Studies on transformation of indica rice with bt - toxin gene mediated by agrobacterium tumefaciens precultured immature embryo and callus derived from young panicle , immature embryo and mature embryo were used as acceptor for genetic transformation mediated by agrobacterium tumefaciens , the transformation rate of the above acceptor was investigated respectively . the results showed that immature embryo after precultured for 4 ~ 6d was the best . in respect to the concentration of agrobacterium tumefaciens when calli were cotransformated in medium yeb , to agrobacterium tumefaciens eha 105 , od value of 0 . 8 was the best 采用农杆菌介导法将bt毒蛋白基因导入水稻同样以上述两种籼稻为主要研究材料，比较了分别以预培养的幼胚和幼穗、幼胚、成熟胚来源的愈伤组织作为转化受体的愈伤组织转化频率，结果表明预培养4 6天的幼胚最适宜作为农杆菌介导转化的受体；其次是来源于幼胚和成熟胚的生长状态良好的胚性愈伤组织。
To set up an efficient plant regeneration system and select wheat genotypes suitable for tissue culture , factors affecting tissue culture were studied with the immature embryos of 24 elite wheat varieties under various conditions including four callus induction media and three differentiation media 摘要为了筛选适合组织培养的小麦基因型，建立一套有效的小麦诱导再生体系，以24个小麦品种的幼胚为研究材料，选用4种诱导培养基和3种分化培养基，研究了影响小麦组织培养的各种因素。
4 . the expression vector was introduced into young embryo - derived calluses of weeker winter - type wheat variety " youngmail58 " by agrobacterium - mediated and biolistic transformation , and 15 gus - positive plant lines were regenerated . the transformation rates by agrobacterium - mediation and biolistic bombardment were 1 . 23 % and 1 . 71 % , respectively 将所构建表达载体分别用农杆菌介导法和基因枪法导入半冬性小麦品种“扬麦158 ”的幼胚愈伤，共获得15株gus阳性苗，其中农杆菌法的转化效率为1 . 23 ，基因枪法的转化效率为1 . 71 。
Based on cdna of tps1 gene , pcambia1303 plasmid , we have succeeded to construct plant reombinant vector phfsm001transforming the construct plant expressing vector to immature of wheat embryo by gene gun , the wheat plant possessing the tpsl gene is attained by tissue culture and hygromylin selected 本文以tps1基因的cdna ，质粒p ~ ( cambia1303 )为研究对象，运用基因克隆技术，成功构建了植物表达载体phfjm001用基因枪转化小麦幼胚，经组织培养和潮霉素筛选得到了含有海藻糖合成酶基因的转基因小麦植株。
