6 . oocytes were fixed for immunofluorescence . examination of cgs and microtubules were performed by fitc labeled lens culinaris agglutinin ( lca ) and and - fi - tubulin under confocal scanning laser microscopy ( cslm ) respectively 利用直接免疫荧光染色和共聚焦显微镜（ confocalscan muglasermicroscopy ， cslm ） ，研究各组体夕成熟卵的皮质颗粒和微管
This study were designed to investigate development potency of the reconstructed embryos of black bear - cat inter - species nuclear transfer in vitro / vivo , to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm . somatic nuclear transplantation technology has been used to establish stem cell from different animals , which can provide materials to treat various diseases 本研究运用体细胞核移植的原理和技术，以六月龄雌性黑熊的成纤维细胞为供核体，去核未受精家猫成熟卵母细胞为受体，构建种间核移植重组胚胎，研究种问体细胞核移植胚胎的构建方法和重组胚胎的体内外发育潜能及其影响因素。
This study were designed to investigate development potency of the reconstructed embryos of rat - mouse and rat - bovine inter - species nuclear transfer in vitro / vivo , to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm . somatic nuclear transplantation technology has been used to establish stem cell from different animals , which can provide material to treat various diseases 本研究运用体细胞核移植的原理和技术，以成年大鼠的成纤维细胞为供体，去核小鼠和牛成熟卵母细胞为受体，构建种间核移植重组胚胎，研究种间体细胞核移植胚胎的构建方法和重组胚胎的体内外发育潜能及其影响因素。
During male gametogenesis , the spermatogonia became primary spermatozoa through mitosis , and then became fourth equivalent cytoplasm , round sperm through meiosis . after maturation , they participated in embryo development with the mature oocyte through fertilization . but in female gametogenesis , the primary oocyte formed a matured egg and 1 ~ 3 polar bodies ( pb ) 雄性动物精子发生过程中精原细胞最后一次有丝分裂形成二倍体精母细胞后，经过两次减数分裂形成了四个含等量细胞质、圆形的精子细胞，最终发育成四个具有正常生理功能的精子，与成熟卵母细胞受精后参与胚胎发育。
The result shows that sperm ultrastructure , spermatogenesis , oogenesis and mature oocyte structure all have the characteristics unique to palaemon modestus . therefore , my researches ca n ' t only enrich the contents of reproductive biology in crustacean but also provide basic references for pond culture in palaemon modestus 结果显示：秀丽白虾精子结构、精子发生过程、卵子发生过程、成熟卵结构均有种的特属性，从而本研究不仅可以丰富甲壳动物生殖生物学内容，同时也可为秀丽白虾人工养殖提供基础资料。
In the presence of hx , oocyte maturation to mil and the extrusion of polar body were highly synchronized , the oocytes matured to mil stage were activated in higher rates than oocytes matured without hx , and those oocytes matured exposed to hx for all the 24h showed a high rate ( 55 % ) of spontaneous activation Hx存在情况下成熟到m期的卵母细胞排出第一极体时同步化程度高，其孤雌活化能力高于正常成熟卵母细胞， hx作用24h组出现自发激活现象(自发活化比率55 ) 。
Techniques in embryo technology ( such as in vitro production of embryos and animal cloning ) need large quantities of high quality oocytes . but the quality of in vitro matured oocytes from slaughtered animals is generally lower than that of the in vivo matured oocytes . it is usually thought that the reason for this poor quality in in vitro matured oocytes is the lack of capacitation during the dominancy of follicular development in vivo 目前胚胎工程技术研究和开发(如体外生产胚胎和体细胞克隆等)需要大量高质量的成熟卵母细胞，但利用屠宰动物卵巢卵母细胞经过体外成熟培养而获取的卵母细胞质量还远不如体内成熟卵母细胞，其原因一般认为是由于缺乏体内主卵泡阶段的获能作用。
