The nucleic acids of the all influenza viruses conducted in the research were extracted from the viruses propagated in specific - pathogen - free chicken embryos . all of the eight segments were amplified by rt - pcr , and the purified pcr products were done cycle sequencing with specific primers , furthermore , the sequencing products were purified and run gel on abi prism 377 dna sequencing machine . the specific primers of the eight genes for pcr and cycle sequencing were designed using the ohgo ( 4 . 0 version ) and genedoc ( 2 . 3 version ) software 首先将实验用毒株在spf鸡胚中增殖，提取核酸，然后应用oligo ( 4 . 0版本)和gendoc ( 2 . 3版本)软件设计h9n2aiv所有8个基因片段特异的pcr引物及序列测定引物，通过rt - pcr方法扩增所有毒株的各个基因片段，纯化后用特异引物进行测序反应，反应产物纯化后在abiprism377dna序列分析仪上进行序列测定，然后应用wisconsinsequenceanalysispackage ( gcg ， 10 . 2版本) 、 phylogeneticanalysisusingparsimony ( paup ， 4 . 0版本)和treeview ( 1 . 5版本)软件进行序列的数据编辑、序列翻译、进化树绘制和遗传演化分析。
This paper presents the overall structure , design idea and systematic function and application condition of information analysis and management system on hydrology and sediment of the yangtze river and mainly introduces function realization of 8 sub - systems of editing sub - system of vector data , management discipline of object relation database , computation sub - system of hydrology and sediment sub - system , visualized analysis sub - system of water and sediment information , analysis sub - system of the yangtze river channel evolution , inquiry sub - system of fundamental information , 3 dimensional visualized sub - system of the yangtze river , network publication sub - system of hydrology , sediment and river channel information 摘要阐述了长江水文泥沙信息分析管理系统的总体结构、设计理念和系统功能以及系统应用情况，重点介绍了矢量数据编辑子系统、对象关系数据库管理子系统、水文泥沙专业计算子系统、水沙信息可视化分析子系统、长江河道演变分析子系统、基本信息查询子系统、长江三维可视化子系统、水文泥沙河道信息网络发布子系统等8个子系统的功能实现。
This paper studies the two problems above . on one hand some necessary improvements are made in existing topology building methods for its higher efficiency and adaptability . on the other hand , a set of methods of updating topology partially are proposed which can reduce the time of rebuilding topology greatly in editing data 本论文重点研究了拓扑关系的建立及其更新问题，一方面对传统的拓扑建立进行了必要的改进，使其具有高效性和通用性；另一方面，提出了一套数据编辑时进行拓扑关系局部更新的方法以减少拓扑重构的时间。
I think this model will provide a great help in vast - producing of spatial data , and will draw more attention in this research field . in order to test the rationality of this model , i processed the scanned map data and autocad data by using the module function i designed in mapinfo . the final result proved that this model could aid us to process spatial data by improving the producing efficiency 为了检验模型设计的合理性，笔者在mapinfo环境中，自行开发了一些工具模块，并结合系统提供的数据编辑功能，对地图和autocad数据进行了矢量数据生产的初步试验，结果表明，模型中对各阶段提出的优化方案，对于提高空间数据的生产效率是有帮助的。
