The products were used in rflp analysis . we found the g849a nonsense mutation that was responsible for the se enzyme inactivation in shandong han chinese and mongolian of inner mongolia individuals was in the same frequency , 0 . 55 % 结果在两个人群中均发现了一个携带g849a点突变的个体，该点突变产生的无功能等位基因se ~ ( 849 )在两个人群中的频率均是0 . 55 。
Pcr method was used to identify candidate ms 188 . sequence analysis indicated that a point mutation occurred in the second exon of the atmyb103 gene in male sterile mutant with caa ( gln ) replaced by a stop codon taa 利用pcr的方法从突变体中扩增atmyb103基因并进行序列分析，结果表明突变体中atmyb103基因第二个外显子上发生了点突变，由原来编码谷氨酰胺的caa密码子突变为终止密码子taa 。
The optimal course is automatically implemented with matlab . the cusp catastrophe model is established by means of the upper bound theorem of plastic limit theory . the characteristic formula of the failure of slope is also deduced 根据分析边坡稳定性的塑性极限方法的上限理论建立了边坡失稳尖点突变模型，并得出边坡突发式滑坡的特征关系式，用突变理论对非饱和土边坡稳定进行了初步研究。
Mbl deficiency and low levels of serum mbl are strongly associated with the presence of variant mbl genes , encoding three different structural variants of the mbl polypeptide , which are caused by three point mutations , cgt52tgt , ggc54gac and gga57gaa , of mbl gene 已发现3个可引起免疫缺损的mbl结构基因点突变? ? cgt52tgt 、 ggc54gac和gga57gaa ，其编码产物氨基酸均发生改变，可导致血清mbl水平低下。
And pcr - rflp analysis was a sensitive , rapid and simple assay for the detection of nucleotide sequence change in ser 83 . and our data sugget that pcr - rflp may be a useful device for detecting the mutation in gyra gene associated the resistance to fqns 本研究通过试验条件的筛选，建立了pcr - rflp检测猪源致病性沙门氏菌gyra基因点突变的方法，可用于沙门氏菌氟喹诺酮类耐药性的分子水平检测和流行病学监测。
The paper uses catastrophe theory to construct the models of the key supporting rock mass that made of different kinds of rock , and discusses the critical requirement of fail . on the critical condition , if the system has small disturbance , the whole stable system will fail 应用突变理论，建立了关键承载层由一层完整岩体构成、一层厚断裂岩体构成、层状岩体构成及软弱夹层滑移的尖点突变失稳模型并得出了失稳的力学判据。
The high - enzyme activity has 2 base changes , resulting in long amino acid sequence with native amylase . this inducing method resolved the problem of non - effective induction as in base analogue induction . and the method we used provide a new measure for this kind of work 高酶活编码区位点突变导致c -端序列变化和终止子的后移本诱变方法克服了用碱基类似物在体内诱变由于核酸复制酶等的校正作用而造成诱变无效的难题，为基因的诱变找到了一条新途经。
A recombinant pbv220 - hil18 - ile48met was constructed and transformed into dh5 ( . the protein expression of the mutant hil18 - ile48met which was induced with different degree of temperature , was observed as a band about 18 kd on sds - page , and its amount was up to 25 % of the total proteins 以重叠延伸法实现点突变，由异亮氨酸突变为蛋氨酸，获得突变体hil - 18 - ile48met ，克隆到表达载体pbv220 ，转化到大肠杆菌dh5 ,温控诱导表达。 sds - page出现了约18kd左右的蛋白条带，表达量占细菌总蛋白的25 % 。
Phase space reconstruction technique is used to reconstruct the phase plane . the approach to detect the abnormal point of the tune - dependent of the crack by phase space is developed . combined with gray system and catastrophe theory , according to observation data of dam cracks , a gray cusp catastrophe model is established to diagnose the abnormality of the crack ( 5 )利用相空间重构技术重构裂缝时效变形的相平面，提出了裂缝时效转异点的相平面识别法；并且基于突变理论和灰色系统理论，建立了裂缝转异诊断的灰色尖点突变模型；并将以上方法和模型用于工程实际，由此对所提方法的可行性进行了验证。
Human elongator complex serves a role in chromatin remodeling through modifying the lysine residues of histone h3 and h4 during transcription elongation in higher cells . the main results and conclusions are as followings . 1 using depletion strain of yeast as material , we have established a system for complementation test and gene expression analysis of human elongator complex 4 、利用点突变和plasmidshuffle等技术构建了含h3k14一r 、 h4ks ~ r单突变和h3k14一侧h4ks一r双突变的e1p3乙菌株，通过功能互补实验分析少凡p3对突变株的补偿功能，证实酵母elongator可对h314k / h4sk进行乙酞化修饰。
