After one month , the transparency of cornea was resumed in 61 patients ( 72 eyes ) , localized leucoma in 27 patients ( 33 eyes ) , corneal opacity and pannus in 11 patients ( 13 eyes ) , full leucoma in 5 patients ( 6 eyes ) , resulting in intraocular infection and evisceration eventually 术后1月有61例（ 72眼）眼角膜恢复透明， 27例（ 33眼）遗留局限性角膜白斑， 11例（ 13眼）角膜浑浊并血管翳， 5例（ 6眼）发生完全性角膜白斑， 2例（ 2眼）角膜自溶穿孔，发生眼内感染，最终行眼内容物摘除。
In this paper , a field strain of infectious bronchitis virus was isolated from proventriculus tissue , morphological observation by electron - microscope and the biological characterizations of the virus were studied , pairs of specific primers are designed and synthesized in correspondence with them , according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes , the cdna of si gene , s2 gene , m gene . n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported 在此基础上，根据国内外已发表的ibv基因序列，分别设计特异性引物，应用不同引物进行反转录合成cdna ，分片段对ibv的主要结构基因进行pcr扩增，并分别将各个目的片段克隆到puc19载体上，在大肠杆菌dh5中实现目的基因的分子克隆，经蓝白斑筛选、限制性内切酶分析、 pcr鉴定，筛选出重组阳性质粒，并对各个目的基因片段进行序列测定，从而获得ibv主要结构基因全序列。
