Collection and preservation of samples : as soon as the three vital signs disappeared , the dogs were anatomized , and the heart , liver , kidney , spleen , lung , brain , muscle in the injection location and no injection location , the heart blood , urine , bile , cerebrospinal fluid ( csf ) in the lateral ventricle and spinal subarachnoid space , spinal cord ( medulla oblongata , cervical cord , the upper beast spinal cord , breast spinal cord and waist spinal cord ) were taken out , some of which were preserved at - 20 for qualitative and quantitative analysis , and the others were fixed with 4 % formaldehyde for the pathology observation 3 、样品采集：当心电、血压和呼吸全部消失时，迅速解剖动物，采取心脏、肝脏、肾脏、脾脏、肺脏、大脑、注射部位肌肉、注射部位20cm以外肌肉、心血、尿液、胆汁、侧山西医科大学硕士学位论文脑室脑脊液、脊髓腔脑脊液和不同节段的脊髓(包括延髓、颈髓、上胸部脊髓、胸部脊髓和腰部脊髓)等组织，冷冻保存。 4 、病理观察:采取心脏、肝脏、 ’肾脏、脾脏、肺脏、大脑、脊髓等组织， 4 %甲醛固定，石蜡包埋，切片， he染色，光镜观察。
For example , when the criminal is released on bail for medical treatment , he would offer the false pathology slices of organize , or others ' malignant tumour pathology information ; and the policyholder offers the fake pathological material while setting a claim , or in the pathology room , we often meet the mark mistake of the sample that is needed to confirm the tissues source of the material . to determine these special material in practice is more and more important 例如：罪犯保外就医提供的是否本人的病理组织和切片，或者是保险理赔时提供的他人的恶性肿瘤病理切片，或是病理室工作中偶尔遇到标本的标记错误，发现与临床资料不匹配时等案件都需要对这种特殊检材的组织来源进行鉴定，石蜡包埋组织或者切片的dna分型鉴定在实践中已经愈发重要。
