It is known from studies using brefeldin a ( bfa ) that disruption of tgn can block the cytotoxicity of ricin , suggesting that the intracellular compartment may be an important part of the uptake pathway . ricin enters the cells by receptor - mediated endocytosis , followed by translocation across the membranes of intracellular organelles . in this study , a trans - golgi retention peptide signal yqrl was fused to the c - terminus of ricin a chain ( rta ) by polymerase chain reaction 本实验为重点研究反式高尔基体在毒素细胞内转运过程中的作用，我们设计了一个反式高尔基体的靶向信号肽基因yqrl ( tyr - gln - arg - leu )连接在rta分子的羧基端，用原核表达蛋白体系，纯化并测定rta - yqrl与rta蛋白对hela (人宫颈癌细胞) ， wish (人羊膜上皮细胞) ， skov - 3 (人卵巢癌细胞)三种细胞的毒性作用。
We showed here that axudl mrna expression was elevated by tgf - 1 treatment in a time and dose - dependent manner in hepg2 hepatoma cells and spc - a1 lung carcinomas cells , and also indicated that de novo protein synthesis was required for this response using the protein synthesis inhibitor cycloheximide , suggesting that axudl is a primary target of tgf - signalling 在hepg2和spc - a1细胞中， tgf - 1能明显诱导这两种细胞中的axud1基因的表达，而且表现出时间和浓度的依赖效应。同时，利用第一军医大学博士学位论文蛋白质合成的特异抑制剂还发现， tgf一pl诱导的axudl基因的表达过程需要新的蛋白质的合成，提示axudl基因是tgf一p信号通路的一个主要靶分子。
Elisa results showed that the translation level of il - 1 and tnf - a detected in culture supernatant of oligochitosan contrast group were as 2 . 4 times and 2 times as the level of blank contrast group ( p < 0 . 01 ) , and the translation level of il - 1 and tnf - a genes of experiment group were as 4 Elisa实验结果显示，两种细胞因子分泌也显著增多，加糖对照组il lp和tnf a分泌量分别为空白对照组的2 4倍和2倍b 0 of人实验组几刀p和tnf a分泌量分别为空白对照组的4
