Abstract : six methods for making preparation of chromosomes of spiders , including gonad slice technique , testis squash technique , single embry drop technique , mix embryo - cells drop technique , blood cell drop technique and single embryo - cell squash technique , are summarized in this paper . the karyotypes of 27 species from 22 genus in15 families are analysed 摘要概述了蜘蛛染色体制片的6种方法：生殖巢切片法、精巢压片法、单胚滴片法、混合胚滴片法、血细胞制片法和单一胚胎压片法，并总结分析了蜘蛛目15科22属27种染色体核型。
In the season of genesis , ( 1 ) testis - net ' s conduit was narrow and it was full of epithelial cell in the conduit . ( 2 ) the epithelial lining of efferent duct appeared phalangeal process and it was filled with secretory product in the efferent duct . ( 3 ) epididymal duct was small and round . it was full of secretory product in the epididymal 生殖殖季节时，精巢网的管腔狭长，腔内充满上皮细胞;输出小管的豁膜上皮呈指状突起，腔内充满脱落的上皮细胞和分泌物;附皋管的管腔较圆且小，腔内充满脱落的上皮细胞和分泌物;输精管的豁膜上皮呈指状突起，肌层明显。
In order to study the regulatory mechanism in gametogenesis of loxoblemmus doenitzi , the special expression of c - kit and c - myc is investigated by immunohistochemical method . the results show that there are kit positive protein granules on the cellular membrane of spermatomeres from metaphase i to anaphase ii , and in the head of mature spermatozoa not only in the testis but also in the spermatheca 结果表明， kit在多伊棺头蟋精子发生中期至后期的精母细胞膜上有阳性颗粒，在精巢和受精囊内精子头部残留的细胞质内也有阳性表达；但在出生后卵子发生过程中，发育中的卵母细胞上无阳性表达。
The testis development stages are determined according to cell ' s morphological characteristics , its size and number and the varying extent of chromatic agglutination . the development of testis may be divided into five stages : ( 1 ) multiplication of spermatogonium ; ( 2 ) growth of spermatocytes ; ( 3 ) maturation of spermatocytes : ( 4 ) initial appearance of spermatozoa ; ( 5 ) complete maturation of spermatozoa ? the reproductive seasons cover from march to april 可根据各期细胞大小、数量多少、核染色质凝聚程度等将精巢的发育时期划分为精原细胞增殖期、精母细胞生长期、精母细胞成熟期、精子开始出现期、精子完全成熟期共5个时期。
In this paper , we selected a widely used organophosphorous pesticide - - monocrotophos , used goldfish as model animal , and chose the vitellogenin induction of male fish as the main indicator , - gtp of sertoli cell in testes , the changes of microstructures of testis and ultrastructures of pituitary and testis as assistant indicators , we finally built up a screening system to effectively detect the environmental estrogen effect of organophosphorous pesticides , and reported that the monocrotophos had the activity of environmental estrogen for the first time in the world 因此，本文选择国内广泛使用的有机磷农药? ?久效磷，以金鱼为模型生物，以雄性金鱼卵黄原蛋白产生、精巢支持细胞- gtp酶活性，并辅以生殖轴线器官? ?垂体和精巢超微结构的变化，在国内首先建立了有机磷农药环境雌激素效应的筛选体系，并首次确认久效磷具有环境雌激素活性。
The full length cdnas of 17 - hsd1 and 17 - hsd3 , 17 - hsd8 were obtained by library screening and race , respectively . expression patterns ( tissue distribution ) of three types 17 - hsds were checked by rt - pcr and northern blot . the recombinant constructs of 17 - hsdl / pet blue2 and 17 - hsd8 / pcdna 3 . 1 were made and subsequently transformed into the corresponding host expression cell of ( de3 ) placi and mammalian hek 293 cell 首先从genebank下载在其他脊椎动物中已被克隆17 - hsd1 ， 17 - hsd3的氨基酸和核甘酸序列，并在序列保守区域设计简并引物，然后分别以罗非鱼卵巢和精巢cdna为模板进行rt - pcr扩增得到17 - hsd1 ， 17 - hsd3的中间片段。
The morphological feature of the somatic cells in the testis of siniperca kneri , such as fibroblasts , sertoli cell and leydig cell are also described in the current paper , meanwhile , both of sertoli cell and leydig cell possess some structural characteristics of typical endocrine gland cell and we have discussed their functions respectively 理学硕士学位论文:大眼撅精巢及精子发育大眼鳃精巢内体细胞包括成纤维细胞、支持细胞、 leydig细胞等。支持细胞、 leydig细胞具有典型内分泌细胞的结构特点。
The testes of pseudobagurs fulvidraco ( richardson ) can be divided into two parts . the anterior part is spermatogenic part where spermatogenesis proceed . the posterior part of it is the seminal vesicle , which can store sperms and produce large amount of fluid during spawning season . at the end of april , the spermatogenic part of the testes , which is at stage , begin the initiation of spermatogenesis . in may , the spermatogenic part of the testes , which is at stage ， starts active spermatogenesis . from june to novermber , the spermatogenic part is at stage , and spermatogenesis is actively proceeding . from december to the beginning of next april , the spermatogenic part is at stage . the wall of the lobulus is made up of cystes of spermatogonia throughout the whole winter time 黄颡鱼的精巢从外形上分为上、下两段.上段为生精部，下段为贮精囊.性成熟黄颡鱼精巢的生精部为小叶型结构，其周年变化经历如下过程：期?期?期?期?期. 4月下旬开始生精活动的启动.生殖季节为5 7月， 5月生精部处于期. 6 7月生精部处于期，产生大量精子. 8 11月虽非生殖季节，生精部仍进行生精活动. 12月至翌年4月，生精部处于期，并以期精巢过冬.贮精囊在生殖季节分泌活动旺盛，产生大量分泌物
