Construction and packing of replication - defective adenovirus vectors expressing hcv structural proteins will play an important role in research of hcv and vaccination . hcv infection often result in persistent infection 构建并包装表达hcv结构基因的复制缺陷型腺病毒载体，在丙型肝炎的防治和hcv的研究中具有重要意义。
Introducion : functional genomics is to analyze the function of genes systematically by using the information of structural genomics , developing and utilizing new experimental tools 前言功能基因组学( functionalgenomics )是利用结构基因组所提供的信息和产物，发展和应用新的实验手段，在基因组或系统水平上全面分析基因的功能。
Regulator gene a gene whose product can promotor or prevent the transcription of structural genes , which may or may not be adjacent to other regulator genes , and may even be on another chromosome 调节基因：这种基因的产物可以促进或抑制结构基因的转录，其可能与其他的调节基因相连，也可以不相连，甚至两者可以不在同一染色体上。
These results further the proof that the prrsv prevalent in china belongs to those of vr - 2332 . however , the proteins encoded by these structural genes show some difference , especially in the composition of amino acid 但从dq和sd株结构基因编码的蛋白来看，这两个毒株与ch - 1a株的结构基因所编码的蛋白在氨基酸组成以及等电点还有一定的差异。
A pair of primer designed according to multi - sequence comparing results of published ibv sequences in genbank was used to amplify the nucleocapsid gene of 5 strains , the amplified rt - pcr product of all 5 strains is 1 . 2kb in length 对3个克隆质粒中的目的基因片段进行序列测定，结果表明该片段含有ibvn基因全序列，其结构基因全长1227bp ，编码409个氨基酸。
China is a populous country with many nationalities , it is necessary to study the genotypes and frequencies of alleles of the exon 1 of mbl gene in chinese so as to provide decision - making evidence for prevention and treatment of mbl deficiency 我国是一个多民族人口大国，有必要对各民族人群mbl结构基因的突变情况进行调查，以便为我国mbl缺损的防治(研究)提供决策依据。
Now , it has been shown that mbl deficiency and low levels of serum mbl are strongly associated with the presence of three variant alleles b , c , d in mbl structural gene , which codons 54 , 57 and 52 amino acid of mbl polypeptide respectively 现已证实， mbl血清浓度低下主要与其结构基因第一外显子的b 、 c和d等位基因变异体(分别编码54 、 57和52位氨基酸)有关，而正常的mbl等位基因为a 。
Mbl deficiency and low levels of serum mbl are strongly associated with the presence of variant mbl genes , encoding three different structural variants of the mbl polypeptide , which are caused by three point mutations , cgt52tgt , ggc54gac and gga57gaa , of mbl gene 已发现3个可引起免疫缺损的mbl结构基因点突变? ? cgt52tgt 、 ggc54gac和gga57gaa ，其编码产物氨基酸均发生改变，可导致血清mbl水平低下。
Blastn analysis showed that each of the 10 sequences had no homology with the structural genes or regulatory genes in the anthocyanin biosynthesis . it was suggested that there were some limitations to isolate the specific ast gene by ddrt - pcr Blastn分析表明，这10个差异表达的cdna片段与数据库中花青苷生物合成途径中的结构基因和调节基因序列没有同源性，表明用ddrt - pcr的方法克隆特定的ast基因有一定的局限性。
The promoter and structure genes of x phage lysis genes were amplified by pcr , respectively . the plasmid mvps was constructed for integrating the vhb gene and the lysis genes into the chromosome of e . colihms174 by ligating the lysis genes to mini - tn5 利用pcr扩增得到噬菌体裂解基因的启动子和结构基因( srrz ) ，连接在转座子minitn5上，构建成为质粒mvps ，用于将vhb基因和srrz基因共同插入到e . colihms174的染色体上。
