Based on the amplitude and phase of two - order cyclic spectrum of phase - code signals , a novel cyclic spectrum estimation approach is proposed to estimate the carrier frequency , chip width , amplitude , initial phase , timing epoch of spreading code under no parameter information . this approach is computationally efficient , and is easy to be implemented , and it shows a good estimation accuracy over analysis 基于相位编码信号循环谱的幅度和相位特性，提出了二阶循环平稳参数估计方法，可庄没有任何参数信息情况下，实现载频、码片时宽、幅度、初相、编码序列初始时间等参数的估计，估计方法计算量少，易于实现，且有良好的估计精度。
Then , a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer . combined with the opuaa - up , a 2 . 3 kb fragment was obtained through pcr . blast result showed a fragment which contained the partial opuaa , the whole opuab and partial opuac sequences were obtained 再次，根据甘氨酸甜菜碱atp转运系统底物结合蛋白的氨基酸保守序列设计下游简并引物，与atp结合蛋白的上游简并引物组合，经pcr扩增获得2 . 1kb的条带，测序后通过blast比较，结果显示获得atp结合蛋白基因的部分序列、通透酶的全部编码序列和部分甘氨酸甜菜碱结合蛋白基因的核苷酸序列。
The method of ctab was specifically improved for the extraction of genomic dna and total rnas from brassica oleracea l . and brassica napus l . 2 . arclboler gene was amplified by pcr with template genomic dna and cdna from roots , leaves , petals , stigmas and ovaries during bud stage in brassica oleracea l . ( 200110197 ) and brassica napus l . ( y578 - 2 ) 以甘蓝( 200110197 )和油菜( y578 - 2 )自交不亲和系的植株为材料，提取幼苗中的gdna及花蕾期的柱头、子房、花瓣、叶片、根的mrna ，并对mrna进行反转录合成cdna ，然后分别以gdna与cdna为模板扩增arc1蛋白编码序列。
Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library . the products were cloned into puc19 vector and their sequences were analysed . the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region , with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv 方法以从噬菌体抗体库中筛选获得的抗hbsag的fab抗体基因为模板，分别扩增出其轻、重链可变区( v _ l 、 v _ h )基因，通过重组pcr方法将轻、重链可变区基因用连接肽( gly _ 4ser ) _ 3的编码序列连接，并引入前导肽编码序列，构建具有l - v _ h - linker - v _ l结构的单链抗体基因。
By cheeking the transformed bacterial colonies , we had filtrated the masculine clone successfully . the sequencing result showed that the inserting fragment contained intact coding sequences of dh . pban ( pheromone biosynthesis activating neuropeptide ) and three sgnps ( suboesophageal ganglion neuropeptide ) and the leading frame of it was correct 测序结果表明，重组载体的插入片段中含有dh 、性信息素生物合成激活肽( pheromonebiosynthesisactivatingneuropeptide ， pban )及三个食道下神经节肽( suboesophagealganglionneuropeptide ， sgnp )的完整的编码序列，且其阅读框架( readingframe )完全正确。
The work of this dissertation is focused on research of some key technics in signal design and processing of mcpc signal . the main content of the dissertation is summarized as follows : it gives a detailed description of the form of the mcpc waveform and its mathematical model , and analyses the formation and properties of phase coded sequence . it discusses the effect of signal parameters on autocorrelation , power spectra and ambiguity function in forms of single pulse and pulse train and compares different kinds of single pulse signals and pulse train signals 本文围绕着多载波相位编码信号在信号设计与信号处理的若干关键问题进行了研究,主要做了以下工作:描述了多载波相位编码信号( multicarrierphasecoded ,简称mcpc )的信号形式,给出了其数学模型,对其中相位编码序列的构成方式与特性进行了分析;从单脉冲和脉冲串两种信号形式入手,对多载频相位编码信号的参数在自相关函数、功率谱密度和模糊方程上的影响作了详细地讨论,对不同调制方式的单脉冲和脉冲串信号进行了比较。
Xylanase refers to a type of enzyme which can hydrolyze xylans into xylooligosaccharides and d - xylose . it broadly exists in microorganism and has wide commerical application in industrial processes , such as feed , paper , foodstuff , medicine and energy industries . the xylanase gene xynb encoding the native protein of xylanase from streptomyces olivaceoviridis a1 was cloned into pichia pastoris expression vector ppic9 a 我们将来源于橄榄绿链霉菌a1 ( streptomycesolivaceoviridisa1 )的木聚糖酶基因xynb的成熟蛋白编码序列克隆到毕赤酵母表达载体ppic9中，转化pichiapastorisgs115得到重组子，实现了木聚糖酶基因xynb的高效表达，表达产物能有效分泌且具有正常的生物学活性。
