The tall fescue seedlings were treated by eight salt levels . we got some important information of the law about growth and partitioning of tall fescue under different salt conditions 本试验设置了8个nacl水乎，探讨了高羊茅在不同盐分水平下其生长和矿质养分吸收、分配的变化规律。
The thesis approve research objects have representative character and universality after systematic analysis of zoology adaptability of poa pralensis , red fes cue and target percnnia ryegrass 本文在系统地分析了黑麦草、紫羊茅和早熟禾的生态适应性后，进一步证实了研究对象具有典型性和普遍性。
The main results are as follows : ( 1 ) plant regeneration system of tall fescue embryogenic calli was established according to studies on impacts of manifold factors on callus induction from mature seeds and subculture production and differentiation of embryogenic calli 主要研究结果如下： ( 1 )通过对成熟种子愈伤组织诱导、胚性愈伤组织继代发生和分化的多种影响因素的研究，建立了高羊茅胚性愈伤组织植株再生体系。
The results showed that tall fescue traple is the hest , tall fescues melike is the second , red clove redquin is the third , award of kentucky bluegrass the worst and the others in the middle in introduced 9 species , according to soil and water conservation and forage grass 结果表明，在参试的9个品种中，以翠碧高羊茅的综合性状最佳，第二为迈洛克高羊茅，第三为雷得昆红三叶，草地早熟禾的优异和新哥来德最差，其它品种居中。
The soil nutrient contents of total nitrogen , total phosphor us and total potassium were studied at the same time . and then , the probable correlations between the quantities of microorganisms and soil nutrient content were analyzed . the results suggested that soil water content decreased with the depth increasing , this value of soil water was the highest in plot g1 ( coniferous forest ) , because of the highest altitude 同一林型下不同土壤深度，养分全量差异显著，且随深度增加而显著减小，不同林型植被之间，全磷含量差异显著，全钾含量在高山柳灌丛( l _ 3 )与洗澡塘沟羊茅群落( l _ 4 )之间差异显著，说明植被类型的不同，对土壤养分全量也有一定的影响。
Bap performed more important function than kt in differentiation of tall fescue embryogenic calli , but better results could be achieved with combination of 2mg / l bap and 0 . 5mg / l kt . at this cytokinin level , 0 . 5mg / l naa was recommended to obtain the highest callus regeneration frequency . plant regeneration could be evidently boosted when embryogenic calli were pre - differentiated on high - osmoticum medium with 60g / l sucrose , and / or when the pre - differentiated compact calli were differentiated on differentiation medium solidified with l0g / l agar 高羊茅胚性愈伤组织分化时， bap的作用要比kt大，但2mg lbap与0 . 5mg lkt配合可获得更佳的效果；在该细胞分裂素水平下，生长素naa用0 . 5mg l ，愈伤组织再生率最高；胚性愈伤组织先在含60g l蔗糖的分化培养基上高渗预分化，以及经高渗预分化后的致密愈伤组织在琼脂浓度为10g l的分化培养基上分化，能明显促进愈伤组织的植株再生；在分化培养基中添加脯氨酸导致愈伤组织再生率下降，但同时有减少白化苗再生的趋势。
Tall fescue ( festuca arundinacea schreb . ) is one of the major cool - season turfgrasses widely utilized at home and abroad , and improvement of its stress tolerances by genetic engineering is of momentous significance to maintaining of perennial evergreen turf , saving of water resource , enlargement of establishment area , and especially , to melioration of ecological environments in western regions of china 高羊茅是目前正在国内外广泛应用的主要冷季型草坪草之一，利用基因工程技术改良其耐逆性对保持草坪四季常绿，节约水资源，扩大建植区域，尤其是对改善我国西部地区的生态环境具有十分重要的意义。
Taken two heavy metal contaminated soils in copper mine field of hongtoushan area and lead - zinc mine field of qingchengzi area as sampling sites , studied the abundance and diversity of amf community and the relation of which to ecological factors ; two experiments were managed by pot culture and four - compartments culture system with festuca . rubra and red clover as host plants inoculated with gintraradices , gmosseae and gcaledordinum to study the effect of different amf on the uptake and accumulation of heavy metal in cd contaminated soil and cu > zn - pk cd complex - contaminated soil 采用盆栽、四室根箱培养的方法，研究了接种g . intraradicesg . mosseae和g . caledordinum在cd污染和cu 、 zn 、 pb 、 cd复合污染土壤中对紫羊茅、三叶草吸收、累积重金属的影响，明确了丛枝菌根菌在重金属污染土壤生物修复中的作用。主要结果如下： 1 、沈阳矿区重金属污染土壤中，共发现丛枝菌根菌6个属23个种，已鉴定22种， 1个未定种。
An experiment has been conducted for investigating the rule of water consumption with three species of turfgrasses : kentucky bluegrass ( poa pratensis ) , perennial ryegrass ( lolium perenne ) , and tall fescue ( festuca arundinacea ) ; two kinds of irrigation water resources : tap water and sewage effluent ; and two kinds of irrigation methods : subsurface dripping irrigation and subsurface leaky pipe irrigation 本研究以三种典型的冷季型草坪草草地早熟禾、多年生黑麦草、高羊茅为供试草种，以自来水和经过砂过滤和沉淀处理的城市居民社区的生活污水为灌溉水源，采用地下滴灌和渗灌两种灌水方法，通过田间对比试验，探讨了污水灌溉条件下草坪草的耗水规律。
In this dissertation , influences of various factors on plant regeneration and agrobacterium - mediated transformation of tall fescue embryogenic calli were systematically studied , and thereafter , stress tolerance - related cbf1 gene guided by constituent promoter camv 35s was incorporated into genome of this grass to obtain transgenic plants with increased stress tolerances 本论文在对高羊茅胚性愈伤组织植株再生与农杆菌介导遗传转化的多种影响因素进行系统研究的基础上，将组成型表达启动子camv35s引导的耐逆相关cbf1基因导入该草种的基因组，获得耐逆性增强的转基因植株。
