A pair of primers containing sph i and hind iii restriction sites were designed , according to the poifn - a gene in ddbj / genbank . then poifn - a gene was cloned from porcine genomic dna by pcr 根据ddbj genbank基因库中已登录的猪干扰素基因序列，设计了含sph和hind酶切位点的一对引物，采取聚合酶链式反应( pcr )法，以猪基因组dna为模板进行了poifn克隆。
The series include : hcg test kit , afp test kit , cea test kit , em antibody test kit , as antibody test kit , etc . the series of pcr kits the series of pcr kits has very high sensitivity , excel distinctiveness and accurate results 基因扩增检测试剂系列是采用聚合酶链式反应技术研制而成，具有配套试剂全标本处理简便灵敏度极高特异性强结果准确等优点，适用于医院临床检测和科研。
Newcastle disease virus ( ndv ) strain 695 , a thermostable nature avirulent strain , were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid . referred to the reported sequence of f gene , a pair of primers were designed and synthesized . f gene of ndv b95 strain was amplified by rt - pcr , the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method 利用从国外引进的新城疫热稳定性天然弱毒b _ ( 95 )株接种spf鸡胚繁殖病毒，经处理后提取病毒的基因组rna ，参考国内外发表的ndv融合蛋白基因序列，设计一对特异性引物，经反转录聚合酶链式反应( rt - pcr )扩增出约1700bp大小的特异性片段，将此片段回收纯化后，利用t - a克隆技术将其克隆到pgem - t - easy克隆载体中，再转化大肠杆菌jm109感受态细胞，转化后经分子量比较、 pcr鉴定和酶切分析筛选阳性克隆。
All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene . this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc ) 所有的样本通过聚合酶链式反应?限制性片段长度多态性方法( pcr - rflp )对ahsg基因的第7个外显子内的sac多态性位点进行基因分型，该多态性位点为238号氨基酸密码子中间的碱基c到g的替换，使苏氨酸( thr ， acc )变为丝氨酸( ser ， agc ) 。
Rapd ( random amplified polymorphic dna ) , which bases on the polymerase chain reaction ( pcr ) , is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms . the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna , and a small number of fragments will be amplified when the primer anneals on each strand over a length range . if sequence variation is present at the priming site , then a fragment may not be amplied , so the dna polymorphic can be detected Rapd (随机扩增多态性dna )技术是二十世纪90年代发展起来的一项dna分子多态性检测技术，它建立于聚合酶链式反应( pcr )技术基础之上，利用随机合成的寡聚核苷酸序列为引物(一般为10个bp ) ，分别与dna的两条单链结合，在dna聚合酶的作用下，对基因组的特定区域进行pcr扩增，其电泳结果为不同大小和数目的dna谱带即rapd图谱，可反映基因组相应区域的dna多态性。
One pair of specific primers pai1 and pai2 was designed according to the sequence of ha gene of aiv h5 subtype reference strain . the fragment of ha gene was amplified by rt - pcr using its genome rna as template . the obtained target fragment was cloned into the vector pmd18 - t and th en e . coli was transformed 以其基因组为模板，根据禽流感h5亚型参考毒株ha基因序列，设计合成了一对特异性引物pai1和pai2 ，经反转录-聚合酶链式反应( rt - pcr )扩增出本毒株的cdna ，所得片段连接到载体pmd18 - t后，转化大肠杆菌。
A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin . four oligonucleotide primers , based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1 , were designed to amplify specific dna fragment from different viruses 依据apmv - 1融合蛋白( f )基因裂解位点的核苷酸序列与其毒力的相关规律，分别设计合成了四条寡核苷酸引物，建立了一个可迅速检测不同禽源apmv - 1并可鉴定强、弱毒株的逆转录酶?聚合酶链式反应( rt - pcr )技术。
Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells , we design a pair of particular primers , and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic . the expression plasmid was identified with pcr and dna sequencing . pbv220 - r hpf4 was transformed into e . coli dh5a , bl21 ( de3 ) and induced by increasing the temperature to 42 . we identified the expression protein by sds - page and western - blotting 目的1人血小板因子4 ( hpf4 )原核高效表达克隆的构建2重组hpf4的表达及分离、纯化工艺研究3重组hpf4的特性研究方法根据原核细胞表达真核蛋白的基因表达调控特点，设计合成一对特异引物，在pt7 - 7 - rpf4表达质粒的基础上，应用聚合酶链式反应( pcr )对其cdna进行改造，通过dna重组技术构建成重组hpf4原核表达质粒pbv220 - rhpf4 ，用快速pcr检测法、 dna测序分析，鉴定重组hpf4表达质粒的正确性。
In this study , the whole e2 genes of two strains of classical swine fever virus ( csfv ) isolated from guangxi ( gx ) were amplified by reverse transcriptase polymerse chain reaction ( rt - pcr ) method and seqenced . the e2 gene fragments of csfv were 1090 base pair in length and encoded 364 amino acid residues 研究采用反转录?聚合酶链式反应( rt ? pcr )技术对两株分别从柳州( gxlz )和南宁( gxnn )分离的广西流行猪瘟病毒( classicalswinefevervirus ， csfv )进行e _ 2全基因的扩增、克隆和测序。扩增片段长度为1090bp ，编码364个氨基酸残基。
Strain pseudomonas psuedoalcaligenese ys1 was capable of producing phas containing monomer of hb and mcl has in certain medium . phacl and phac2 , two key polyhydroxyalkanoates polymerase genes of pha biosynthesis were amplified and cloned from chromosomal dna of pseudomonas psuedoalcaligenese ys1 using pcr 本研究利用聚合酶链式反应( pcr )技术，从p . psuedoalcaligeneseys1染色体dna中扩增并克隆了调控短链与中链pha生物合成的两个关键酶基因： phac1 、 phac2基因。
