肝细胞生长因子

• Methods : hepatocytes obtained from mice with in situ portal vein collagenase perfusion were inoculated on two types of medium , which one was 10 % pcs rpmi 1640 medium ( common medium ) , the other was rpmi 1640 medium supplemented with 5 u g / ml tranferrin , 5 u g / ml insulin , 10nm nicotinamide , 5mm - me , 40 u g / ml hgf ( special medium )
  一种是在rpmi1640培养液中加入了511iml转铁蛋白、 5pg ml牛胰岛素、 10nm烟酚胺、 smm卜琉基乙醇， 4011g ml促肝细胞生长因子，简称为肝细胞培养液。